FastQCFastQC Report
Mon 6 Feb 2017
SRR2077310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313270
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.043.221691
GTATCAA51800.040.2969471
TATCAAC71200.029.0597672
TCAACGC71450.028.8113174
ATCAACG72400.028.433273
CAACGCA72400.028.2853685
AACGCAG75250.027.351646
ACGCAGA85650.023.8062177
CGCAGAG88650.022.9517448
GAGTACT60600.021.9672612
AGAGTAC88450.021.95188311
GTACATG61900.020.726051
CAGAGTA94350.020.72537410
AGTACTT65450.020.55020513
GCAGAGT97450.020.4199739
GTACTTT67050.019.74907314
TACATGG65350.019.463382
ACATGGG67700.018.4286173
TACTTTT74600.017.84278915
ACTTTTT79400.017.15648816
Produced by FastQC (version 0.11.5)