FastQCFastQC Report
Mon 6 Feb 2017
SRR2077309_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077309_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895016
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42090.22210894261578792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.10015746568894406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.023.9887051
GTATCAA28950.022.5021841
TTAGTCG550.007760876318.18837239
TATCAAC37050.017.452782
TCAACGC37150.017.2318464
ATCAACG37150.017.1259563
CAACGCA37950.016.9708845
AACGCAG39450.016.3195746
CGTCCGA904.5922858E-415.55089811
CGTGCCG1201.5871155E-514.99550810
ACGCAGA44900.014.0269787
TCGTCCG1000.00102068213.99580810
CGCAGAG45950.013.7064498
GTATAGA3000.013.3628351
GTACATG46750.013.2913771
AGAGTAC44350.013.02879111
GTGTAGC6050.012.9210891
GAGTACT24000.012.8294912
TAGCCCT6250.012.8032624
AGTACTT25150.012.71984813
Produced by FastQC (version 0.11.5)