FastQCFastQC Report
Mon 6 Feb 2017
SRR2077309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895016
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.038.3470841
GTATCAA37050.037.525511
TATCAAC51450.026.7418382
TCAACGC51750.026.5184384
ATCAACG51950.026.3496383
CAACGCA51950.026.348245
CGCGGTA809.5939606E-525.86351231
AACGCAG54100.025.2997886
ACGCAGA64550.021.216857
CGCAGAG66800.020.4951788
GAGTACT46050.019.3968312
AGAGTAC67850.019.0100111
GTGGTAT7900.018.8717371
AGTACTT48500.018.55920613
GTACATG49750.018.5378361
CAGAGTA72250.018.09049810
GCAGAGT73400.017.9945249
GTACTTT50950.017.46185114
TACATGG53300.017.2307742
ACATGGG51900.017.1604583
Produced by FastQC (version 0.11.5)