FastQCFastQC Report
Mon 6 Feb 2017
SRR2077308_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077308_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133492
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.25840265630243753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25230.1182568296482949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24790.11619448303532426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.024.691211
GTATCAA31900.024.3895781
GTATACG550.00766118918.2293241
ATCAACG43200.017.9710313
TATCAAC43300.017.9392072
TCAACGC44150.017.628424
CAACGCA44550.017.419515
AACGCAG46350.016.8649656
CCGATCG750.002449272715.9936839
ATACCGT1651.007902E-714.5397116
ACGCAGA54150.014.1772257
TATACCG1000.001018059214.0007065
ATTTGCG1303.3793585E-513.84133516
CTATACC2201.2696546E-913.6415294
CGCAGAG55950.013.5067278
ACCGTCG1050.001476228613.3280688
AGAGTAC54050.013.24176211
GTATAGT3300.012.7605261
CAGAGTA57200.012.54749110
GAGTACT25450.012.49015412
Produced by FastQC (version 0.11.5)