##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077308_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2133492
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.760023004539036	31.0	31.0	34.0	25.0	34.0
2	29.808419248818367	31.0	30.0	34.0	25.0	34.0
3	29.960314357869635	31.0	31.0	34.0	25.0	34.0
4	33.32430775461075	37.0	35.0	37.0	27.0	37.0
5	33.393733841045574	37.0	35.0	37.0	27.0	37.0
6	33.42544570122597	37.0	35.0	37.0	27.0	37.0
7	33.336941971190896	37.0	35.0	37.0	27.0	37.0
8	33.28544002039848	37.0	35.0	37.0	26.0	37.0
9	34.7291679556333	39.0	35.0	39.0	27.0	39.0
10	34.650362879260854	39.0	35.0	39.0	26.0	39.0
11	34.677716626075934	39.0	35.0	39.0	27.0	39.0
12	34.58852763450718	39.0	35.0	39.0	26.0	39.0
13	34.547141962566535	39.0	35.0	39.0	25.0	39.0
14	35.55012345956769	40.0	36.0	41.0	24.0	41.0
15	35.68749261773655	40.0	36.0	41.0	25.0	41.0
16	35.656924422496076	40.0	36.0	41.0	25.0	41.0
17	35.66846887637732	40.0	36.0	41.0	25.0	41.0
18	35.62536676959651	40.0	36.0	41.0	25.0	41.0
19	35.717434609550914	40.0	36.0	41.0	25.0	41.0
20	35.72028158530709	40.0	36.0	41.0	25.0	41.0
21	35.723233553254474	40.0	36.0	41.0	25.0	41.0
22	35.66417075854983	40.0	36.0	41.0	25.0	41.0
23	35.61422119229882	40.0	36.0	41.0	25.0	41.0
24	35.643012488446175	40.0	36.0	41.0	25.0	41.0
25	35.57379872996946	40.0	36.0	41.0	25.0	41.0
26	35.603451993258	40.0	36.0	41.0	25.0	41.0
27	35.57730846893262	40.0	36.0	41.0	25.0	41.0
28	35.50001499888446	40.0	36.0	41.0	25.0	41.0
29	35.461396621126305	40.0	36.0	41.0	24.0	41.0
30	35.36704895073429	40.0	36.0	41.0	24.0	41.0
31	35.336505128681054	40.0	36.0	41.0	24.0	41.0
32	35.301949573750456	40.0	36.0	41.0	24.0	41.0
33	35.25210359354523	40.0	35.0	41.0	24.0	41.0
34	35.19228288646032	40.0	35.0	41.0	24.0	41.0
35	35.15944845352127	40.0	35.0	41.0	24.0	41.0
36	35.09317869483457	40.0	35.0	41.0	23.0	41.0
37	35.004382486552565	40.0	35.0	41.0	22.0	41.0
38	34.95887587110709	40.0	35.0	41.0	22.0	41.0
39	34.87807922410771	40.0	35.0	41.0	21.0	41.0
40	34.83852435350121	40.0	35.0	41.0	20.0	41.0
41	34.704894135998636	40.0	35.0	41.0	18.0	41.0
42	34.64308279571707	40.0	35.0	41.0	17.0	41.0
43	34.51183411983734	40.0	35.0	41.0	15.0	41.0
44	34.414637598828584	40.0	35.0	41.0	14.0	41.0
45	34.2973013257139	40.0	35.0	41.0	7.0	41.0
46	34.170706053737256	39.0	35.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31837801312766345
1101	2	0.2681429510238651
1101	3	0.3228162461755346
1101	4	0.38862972002195306
1101	5	0.3940134212639421
1101	6	0.23944984741427788
1101	7	0.425515850522423
1101	8	0.5332699002165668
1101	9	0.4784209279712215
1101	10	0.6488524795397836
1101	11	0.5281555482437312
1101	12	0.6391589632028953
1101	13	0.5419919476028099
1101	14	0.6252556653907462
1101	15	0.515955378346753
1101	16	0.4360936316246864
1101	17	0.5205933532676923
1101	18	0.5863393171654252
1101	19	0.4369456368591358
1101	20	0.48083263172495094
1101	21	0.41431709932646044
1101	22	0.5582804946648849
1101	23	0.5225847835193917
1101	24	0.5255625767761885
1101	25	0.5164514448850284
1101	26	0.5883848968272645
1101	27	0.6484066280463949
1101	28	0.4617586744451714
1101	29	0.4588885736179691
1101	30	0.5356022914082814
1101	31	0.5230886955552165
1101	32	0.48267734124280537
1101	33	0.5516866850733564
1101	34	0.43528742038058965
1101	35	0.4852633779937747
1101	36	0.38771345540050817
1101	37	0.4999481267993744
1101	38	0.34643488391013477
1101	39	0.4413990677453583
1101	40	0.47362383408279385
1101	41	0.46644875678538256
1101	42	0.30595420945913077
1101	43	0.34410181459749367
1101	44	0.4206591414956762
1101	45	0.39472625334655476
1101	46	0.3086048704993729
1102	1	0.37407031170590344
1102	2	0.4179150440034114
1102	3	0.4592975597269877
1102	4	0.5424453380855425
1102	5	0.5386112867404478
1102	6	0.5898551813319344
1102	7	0.5177190714719018
1102	8	0.48596264334346273
1102	9	0.5095233931642085
1102	10	0.5920091366052915
1102	11	0.5300792293397336
1102	12	0.6280036113522129
1102	13	0.6605824678555905
1102	14	0.8602377543744666
1102	15	0.7118709552625333
1102	16	0.7507565163564465
1102	17	0.6792169250049582
1102	18	0.7015797225814282
1102	19	0.7806010163677115
1102	20	0.7089810193766084
1102	21	0.6823541226039396
1102	22	0.69677244812015
1102	23	0.7263052405729837
1102	24	0.6880238750509662
1102	25	0.731253618355602
1102	26	0.7527866493743787
1102	27	0.6761475583676457
1102	28	0.7939282348416299
1102	29	0.8135353798921798
1102	30	0.7684720308018953
1102	31	0.7979907063021798
1102	32	0.7764922384166866
1102	33	0.7602763595297972
1102	34	0.8275414723814762
1102	35	0.72196525286612
1102	36	0.7109141248048942
1102	37	0.6297715599735554
1102	38	0.6512065908687035
1102	39	0.6965002843478558
1102	40	0.6402810435330153
1102	41	0.7323590364703634
1102	42	0.6282019007384676
1102	43	0.5459895939586161
1102	44	0.5830069531731112
1102	45	0.4853479184138223
1102	46	0.383506024743717
1103	1	0.4273613631967379
1103	2	0.3662754863607631
1103	3	0.4156607722740233
1103	4	0.3100182788763419
1103	5	0.43362949029764053
1103	6	0.4040605484169433
1103	7	0.4195450746700402
1103	8	0.4292067172592908
1103	9	0.5576014390734869
1103	10	0.5321924344225053
1103	11	0.5376504511369333
1103	12	0.49431822183029084
1103	13	0.48785286127337457
1103	14	0.7290593055141912
1103	15	0.6456620268907898
1103	16	0.6948878744671347
1103	17	0.5581763758810041
1103	18	0.5681956069372589
1103	19	0.6083883453599128
1103	20	0.6442679979326158
1103	21	0.6181161868902549
1103	22	0.5919252011761458
1103	23	0.5960528882629959
1103	24	0.7404133549001557
1103	25	0.656352072363859
1103	26	0.6584375258248656
1103	27	0.6315571119670551
1103	28	0.781341556727007
1103	29	0.8041496523540133
1103	30	0.762573790272846
1103	31	0.8034551484471351
1103	32	0.7392923394863118
1103	33	0.8217999053545881
1103	34	0.8520250936799911
1103	35	0.8143513712278292
1103	36	0.7722809587134378
1103	37	0.8504968627717986
1103	38	0.7546434305664746
1103	39	0.7698489986238926
1103	40	0.704303205050465
1103	41	0.8318929282066136
1103	42	0.7439589070436199
1103	43	0.6712408898975326
1103	44	0.6195184671677225
1103	45	0.4981625206794291
1103	46	0.588812922475519
1104	1	0.6047725660118815
1104	2	0.50513747730297
1104	3	0.5810469687104387
1104	4	0.5802545663867988
1104	5	0.5415927541501091
1104	6	0.5223157098695879
1104	7	0.5339298110868995
1104	8	0.5709318260520106
1104	9	0.5472481905299631
1104	10	0.7168244439338949
1104	11	0.6305045501772923
1104	12	0.651897566097233
1104	13	0.6146309312125666
1104	14	0.779505949241063
1104	15	0.7482237499264954
1104	16	0.6824043971399618
1104	17	0.6858975227759814
1104	18	0.5902205955973017
1104	19	0.6753842399408327
1104	20	0.6613144793563492
1104	21	0.5803773833266703
1104	22	0.6986510364349101
1104	23	0.7447533999785918
1104	24	0.6317823181445164
1104	25	0.4964881252789368
1104	26	0.6244938428900824
1104	27	0.7689371564763903
1104	28	0.7054657221769887
1104	29	0.7266348395341922
1104	30	0.7541346025681932
1104	31	0.6942673020468675
1104	32	0.7520072671232043
1104	33	0.7855634247104817
1104	34	0.8015578519829631
1104	35	0.7789930728593646
1104	36	0.8089331639808464
1104	37	0.7415792263024983
1104	38	0.7085035344688393
1104	39	0.8205926517912872
1104	40	0.8087840830466959
1104	41	0.9766071789897808
1104	42	0.8161609939502199
1104	43	0.7474945753838043
1104	44	0.7505970831967588
1104	45	0.6908897539423577
1104	46	0.628994246628217
1105	1	0.09148073815888225
1105	2	0.12181421811535031
1105	3	0.26736915716028875
1105	4	0.19323898887919455
1105	5	0.3734981492868883
1105	6	0.37654426057420665
1105	7	0.21107128663263097
1105	8	0.31719978681464767
1105	9	0.3057009744481718
1105	10	0.18863727206321101
1105	11	0.2821201553168322
1105	12	0.3352216887830579
1105	13	0.41237547390391427
1105	14	0.601353317298539
1105	15	0.42701709753633565
1105	16	0.5036041415444927
1105	17	0.3365555084073719
1105	18	0.30269728114578953
1105	19	0.38170245629893884
1105	20	0.33341916987858866
1105	21	0.350993402945349
1105	22	0.2910833381757172
1105	23	0.37650250194045753
1105	24	0.2277808674675299
1105	25	0.30689759612723577
1105	26	0.3300824032658767
1105	27	0.4708783004388124
1105	28	0.5595725196348553
1105	29	0.43137717167014245
1105	30	0.558675739622629
1105	31	0.6071299712925153
1105	32	0.5253977064710895
1105	33	0.5899845355145672
1105	34	0.5093396977967259
1105	35	0.6144647155783431
1105	36	0.5830757102643531
1105	37	0.5481867145589163
1105	38	0.4785867756956961
1105	39	0.46442036662963915
1105	40	0.26132276776623087
1105	41	0.4117325589289891
1105	42	0.43337137665263015
1105	43	0.46176820069466373
1105	44	0.44409580521941194
1105	45	0.4093332120799644
1105	46	0.42850464314658865
1106	1	0.08457893431226893
1106	2	0.04001992830529133
1106	3	0.11256345038349025
1106	4	0.06464878801567409
1106	5	0.1482400302705571
1106	6	0.16588074537718
1106	7	0.15787786376115065
1106	8	0.18944452630643127
1106	9	0.0162091915923952
1106	10	0.1470451257075993
1106	11	0.23217604555011917
1106	12	0.04852015116168218
1106	13	0.09870668539938521
1106	14	0.3201251827524061
1106	15	0.2749457457606468
1106	16	0.33981162656932185
1106	17	0.3388256758220862
1106	18	0.22448935241224177
1106	19	0.2226017344189799
1106	20	0.15475771963370022
1106	21	0.2670556586922288
1106	22	0.2805648815994459
1106	23	0.24948192068202957
1106	24	0.29943598633027335
1106	25	0.2933115763651344
1106	26	0.25609909354012217
1106	27	0.3883489047359632
1106	28	0.3501189639920739
1106	29	0.43386322044396763
1106	30	0.5113597507494774
1106	31	0.4039612937151631
1106	32	0.3564820623254903
1106	33	0.38783297485446866
1106	34	0.43427271484537755
1106	35	0.26720803008250016
1106	36	0.2521590251098189
1106	37	0.09281954559122596
1106	38	0.138177710385186
1106	39	0.2463464891273759
1106	40	0.2548778985749749
1106	41	0.502097680010273
1106	42	0.18590606272870502
1106	43	0.38727163943726595
1106	44	0.3911976193054585
1106	45	0.3375398608845117
1106	46	0.1762169833687821
1107	1	0.266401043276435
1107	2	0.20043321965232153
1107	3	0.25173269593835457
1107	4	0.31091498852138244
1107	5	0.31062132667305775
1107	6	0.1625980627519894
1107	7	0.24553668034974407
1107	8	0.30362502406956793
1107	9	0.3874191310567454
1107	10	0.23254894898992262
1107	11	0.34221029998743546
1107	12	0.29893856051496215
1107	13	0.29816904789410614
1107	14	0.4916742518430226
1107	15	0.46913767264062756
1107	16	0.5462937625058757
1107	17	0.46297497184937697
1107	18	0.5916888155975641
1107	19	0.49810557415791124
1107	20	0.4428985915214412
1107	21	0.38499587511313393
1107	22	0.30576427402134243
1107	23	0.262088096683847
1107	24	0.3454887857233544
1107	25	0.23842342784092097
1107	26	0.09197442483228713
1107	27	0.2832958609621983
1107	28	0.23641221093916442
1107	29	0.4314928512316598
1107	30	0.3041134109056358
1107	31	0.27246412054964253
1107	32	0.31064114180845337
1107	33	0.35436763206735833
1107	34	0.23343560814811326
1107	35	0.18960235189319263
1107	36	0.22312462503148822
1107	37	0.25783060854845985
1107	38	0.19351574153261453
1107	39	0.33853437097219796
1107	40	0.22994860029312747
1107	41	0.3336508879387381
1107	42	0.3613302524668569
1107	43	0.2882962280836807
1107	44	0.27578123000127164
1107	45	0.2981476693822174
1107	46	0.4738140300652418
1108	1	0.47352338686819806
1108	2	0.43396348859795353
1108	3	0.445323301781098
1108	4	0.5437239976975405
1108	5	0.5323605831493197
1108	6	0.3633888974188437
1108	7	0.43356206745674086
1108	8	0.4862834808983436
1108	9	0.4218024655496606
1108	10	0.5956077707003828
1108	11	0.487579510776186
1108	12	0.4286640488920881
1108	13	0.44962896115740136
1108	14	0.5921742247861133
1108	15	0.6555564594090271
1108	16	0.6954551917288754
1108	17	0.6356496241681526
1108	18	0.6717849835668304
1108	19	0.582700532297018
1108	20	0.5976447591041776
1108	21	0.6042601978788156
1108	22	0.6817118454708933
1108	23	0.5584649839029368
1108	24	0.7374610021476684
1108	25	0.662769810332783
1108	26	0.5783748515583298
1108	27	0.6405552284490739
1108	28	0.7308367492324166
1108	29	0.7483284302880691
1108	30	0.45487583582272606
1108	31	0.6037629679255119
1108	32	0.6988945799679627
1108	33	0.5657381029348016
1108	34	0.5961763179666804
1108	35	0.5923787768257398
1108	36	0.7219445574665286
1108	37	0.7121958535680619
1108	38	0.5895335449758008
1108	39	0.631247366789971
1108	40	0.6624451022218949
1108	41	0.621476827006596
1108	42	0.5063952663431834
1108	43	0.5528416334347668
1108	44	0.43761031540059037
1108	45	0.4511562039029613
1108	46	0.5018772581858713
1109	1	-0.003316040238075857
1109	2	-0.05158399087297738
1109	3	-0.005889240434797216
1109	4	0.02406720305599208
1109	5	0.058754978459539586
1109	6	-0.025994722248398716
1109	7	-0.036275168376221245
1109	8	-0.029946060628546434
1109	9	0.04588585818292046
1109	10	0.03292154070704356
1109	11	0.030713426943037803
1109	12	-0.06628811583745176
1109	13	0.09533056394958095
1109	14	0.1571852941048988
1109	15	0.09300718399759944
1109	16	0.05879795657362763
1109	17	0.07669335927258203
1109	18	-0.005069974471417993
1109	19	0.08983854862990626
1109	20	-0.03940583220075666
1109	21	0.016328296779789753
1109	22	-0.008223545852118264
1109	23	-0.05045287723358882
1109	24	-0.03803118720545484
1109	25	0.14236414238975925
1109	26	0.15643388039503492
1109	27	0.04378905086557694
1109	28	0.0963278655393296
1109	29	0.13109982626470895
1109	30	0.0846401609896077
1109	31	0.17236535347665694
1109	32	0.15045840362694207
1109	33	0.1624110949264903
1109	34	0.16242901470123172
1109	35	0.21980527316043919
1109	36	0.11736603872211049
1109	37	0.17326206616674966
1109	38	0.14162626746345808
1109	39	0.19484766272481835
1109	40	0.1063129255194255
1109	41	0.12625244739673036
1109	42	0.1870784579214657
1109	43	0.1716368849229113
1109	44	0.07422011061819234
1109	45	0.10548255678832419
1109	46	0.10023521510589006
1110	1	0.04369948141088287
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2216	15	-3.079905647741789
2216	16	-2.668345760499534
2216	17	-2.5133041651167645
2216	18	-2.809514343403656
2216	19	-2.662896667548466
2216	20	-2.5172162224573853
2216	21	-2.606233864826308
2216	22	-2.530134950022976
2216	23	-2.4966941500465794
2216	24	-2.5189797301134647
2216	25	-2.560807657154207
2216	26	-2.4541425551895557
2216	27	-2.7087213354887254
2216	28	-2.804508527783767
2216	29	-2.9694818840540904
2216	30	-2.699256794645443
2216	31	-2.6700889875659755
2216	32	-2.611932935413101
2216	33	-2.5660734550829787
2216	34	-2.5040452510117817
2216	35	-2.4223297288477426
2216	36	-2.7223304518767364
2216	37	-2.6862168111954077
2216	38	-2.709733832721575
2216	39	-2.6362949762823504
2216	40	-2.547341462940736
2216	41	-2.6692037713395678
2216	42	-2.5969288179378225
2216	43	-2.6216133948148865
2216	44	-2.642701529981636
2216	45	-2.752657970230473
2216	46	-2.7698753665664135
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95087.0
3	2972.0
4	8542.0
5	6521.0
6	5118.0
7	4398.0
8	3699.0
9	3389.0
10	3109.0
11	3040.0
12	2951.0
13	2996.0
14	3262.0
15	3304.0
16	3570.0
17	3952.0
18	4360.0
19	5162.0
20	6832.0
21	9375.0
22	11758.0
23	13724.0
24	14722.0
25	15734.0
26	16956.0
27	19528.0
28	21782.0
29	24244.0
30	26741.0
31	32580.0
32	40126.0
33	50586.0
34	64883.0
35	86218.0
36	119442.0
37	173467.0
38	310400.0
39	908962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89471573144161	18.017820415934953	13.797178285413155	24.290285567210283
2	15.499104713362891	20.120359540360123	38.69218741646258	25.688348329814403
3	18.424606102278094	25.177044673088222	29.324244907224795	27.07410431740889
4	12.890007799335251	16.670598382071752	37.64921563450148	32.79017818409151
5	14.265725346617849	37.28214467919093	34.073163000420145	14.378966973771082
6	31.933187469181977	36.61560483939007	17.673232428338142	13.77797526308981
7	28.194903004089074	31.99880758868559	21.84990616322911	17.95638324399623
8	26.78927317280777	33.831577526421476	20.143876799163063	19.235272501607692
9	26.93555916778689	15.902379760505312	19.152731765574934	38.009329306132855
10	15.038162786642745	28.581733608562864	33.19604666902899	23.1840569357654
11	34.32386903724036	23.409274560204587	23.707330517292778	18.559525885262286
12	23.886285957481913	24.66327504391861	29.72961698473676	21.72082201386272
13	27.68353478710021	22.247376601365275	25.92407189715265	24.145016714381867
14	22.739293140072707	21.247232237102367	26.74924489991057	29.264229722914358
15	23.670161406745375	28.7241292678857	23.74492147146556	23.860787853903364
16	23.682957330048577	28.207230212252966	24.920318426317042	23.189494031381415
17	22.571916438987326	27.828719516396426	26.4160529987818	23.18331104583444
18	22.673964327581043	27.556341338917978	27.19537991880829	22.57431441469269
19	23.637555882824415	27.052349164417606	27.30930000815568	22.000794944602298
20	23.837493537399183	26.587292101119843	27.062161431198316	22.513052930282658
21	23.847262923235856	26.366288498784122	27.15421637502555	22.632232202954473
22	23.841570533191593	26.0561089518967	26.98013397753542	23.122186537376283
23	23.465834602381356	26.19671345856482	26.9451588454405	23.392293093613326
24	23.239005531802004	26.445078983558336	26.945950329086443	23.36996515555322
25	23.498796093314603	26.233260259923853	27.054597882980364	23.21334576378118
26	23.32348397354172	26.531681690690107	26.96351299665522	23.181321339112955
27	23.372770384470225	26.644412511398613	26.795612618293575	23.18720448583759
28	23.391026458280212	26.39270634908731	26.87834665746727	23.337920535165214
29	23.450570012496122	26.63637786845981	26.822319778838338	23.09073234020573
30	23.551188096303882	26.561328924209793	26.805158768288994	23.08232421119733
31	23.61917511189607	26.20845174465995	26.80335587425643	23.369017269187555
32	23.400238296787307	26.42155470373672	26.746517651492358	23.431689347983617
33	23.46743250122103	26.377278824962058	26.703792830941115	23.451495842875797
34	23.722235476811317	26.334644678318934	26.776702150946385	23.16641769392336
35	23.633581961177306	26.439468971048207	26.725766753552826	23.20118231422166
36	23.618888471595696	26.435758181877006	26.660815184595975	23.284538161931327
37	23.6963758869969	26.228717465061536	26.775845371678535	23.299061276263032
38	23.558734876220566	26.36771468618011	26.767249663655583	23.30630077394374
39	23.666110014532983	26.60844094727468	26.57401949267278	23.151429545519562
40	23.74714330249467	26.23089382109514	26.871872282474936	23.150090593935246
41	23.576051104533835	26.367318761293784	26.718589738478993	23.33804039569339
42	23.69162555939192	26.358275342294863	26.68652384878526	23.263575249527957
43	23.580548127088672	26.28062914217773	26.731838179861	23.4069845508726
44	23.693284060039403	26.375777943855827	26.46884520640303	23.462092789701742
45	23.641826956887847	26.398868771279695	26.46863287670976	23.4906713951227
46	23.79313497281978	26.23318963799558	26.38574579755601	23.587929591628633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	495.0
1	534.0
2	573.0
3	738.5
4	904.0
5	928.0
6	952.0
7	952.0
8	932.0
9	912.0
10	1077.5
11	1243.0
12	1809.0
13	2375.0
14	4427.5
15	6480.0
16	8926.0
17	11372.0
18	11817.0
19	12262.0
20	12262.0
21	13315.0
22	14368.0
23	17990.0
24	21612.0
25	26769.0
26	31926.0
27	37205.5
28	42485.0
29	47973.5
30	53462.0
31	53462.0
32	60236.0
33	67010.0
34	74812.5
35	82615.0
36	90069.0
37	97523.0
38	104180.5
39	110838.0
40	119417.0
41	127996.0
42	133941.5
43	139887.0
44	139887.0
45	145723.5
46	151560.0
47	156141.5
48	160723.0
49	163021.5
50	165320.0
51	163082.5
52	160845.0
53	155941.5
54	151038.0
55	142449.5
56	133861.0
57	133861.0
58	124585.5
59	115310.0
60	102413.0
61	89516.0
62	77323.5
63	65131.0
64	54709.5
65	44288.0
66	36073.0
67	27858.0
68	22731.0
69	17604.0
70	17604.0
71	13791.5
72	9979.0
73	7950.0
74	5921.0
75	4598.0
76	3275.0
77	2581.0
78	1887.0
79	1404.0
80	921.0
81	921.0
82	731.5
83	542.0
84	404.0
85	266.0
86	220.5
87	175.0
88	123.5
89	72.0
90	53.0
91	34.0
92	29.0
93	24.0
94	24.0
95	20.0
96	16.0
97	14.5
98	13.0
99	18.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20440667225375114
2	0.056902017912417766
3	0.06576073404540538
4	0.05901123603931957
5	0.04143441831513781
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.218436253803624E-4
18	0.0010780448204164815
19	4.687151393115137E-4
20	0.0029529053776625363
21	0.0022498326686952656
22	0.0
23	1.406145417934541E-4
24	4.687151393115137E-4
25	9.843017925541787E-4
26	9.374302786230274E-4
27	0.026107433259651314
28	9.374302786230274E-4
29	0.001406145417934541
30	0.0017342460154526008
31	0.0018279890433149034
32	8.436872507607248E-4
33	0.002156089640832963
34	0.002765419321937931
35	0.002296704182626417
36	0.03276318823787481
37	0.007968157368295732
38	0.01228033664996166
39	0.05160553683819766
40	0.016264415334109526
41	0.05788631970497194
42	0.05933933663683764
43	0.02606056174572016
44	0.029341567720900755
45	0.027654193219379307
46	0.023763857563093745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2133492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.57409781595999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64462283611816	52.226242883708515
2	11.981631265231472	15.713693211621058
3	3.7571450331593983	7.391141877394277
4	1.6378901973407134	4.2961268804888775
5	0.8571457370242817	2.810327920108168
6	0.5146045260140197	2.0246836515227433
7	0.34054307128584643	1.5631563266931867
8	0.21918577754015067	1.1498327693028065
9	0.18142593773978766	1.0707157968930991
>10	0.827991231879141	9.229321930459314
>50	0.030836111406332092	1.341416435000849
>100	0.006762769976105638	0.6880289772808083
>500	0.0	0.0
>1k	1.4367018969506856E-4	0.23562028723827178
>5k	7.183509484753428E-5	0.25969105228800327
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5513	0.25840265630243753	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2523	0.1182568296482949	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2479	0.11619448303532426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.687151393115137E-5	0.0
4	0.0	0.0	0.0	9.374302786230274E-5	0.0
5	0.0	0.0	0.0	9.374302786230274E-5	0.0
6	0.0	0.0	0.0	9.374302786230274E-5	0.0
7	0.0	0.0	0.0	9.374302786230274E-5	0.0
8	0.0	0.0	0.0	9.374302786230274E-5	0.0
9	0.0	0.0	0.0	9.374302786230274E-5	0.0
10	4.687151393115137E-5	0.0	0.0	9.374302786230274E-5	0.0
11	4.687151393115137E-5	0.0	0.0	9.374302786230274E-5	0.0
12	9.374302786230274E-5	0.0	0.0	9.374302786230274E-5	0.0
13	9.374302786230274E-5	0.0	0.0	9.374302786230274E-5	0.0
14	9.374302786230274E-5	0.0	0.0	9.374302786230274E-5	0.0
15	9.374302786230274E-5	0.0	0.0	9.374302786230274E-5	0.0
16	9.374302786230274E-5	0.0	0.0	1.406145417934541E-4	0.0
17	1.406145417934541E-4	0.0	0.0	1.406145417934541E-4	0.0
18	1.406145417934541E-4	0.0	0.0	1.406145417934541E-4	0.0
19	1.406145417934541E-4	0.0	0.0	1.406145417934541E-4	0.0
20	1.406145417934541E-4	0.0	0.0	1.406145417934541E-4	0.0
21	1.406145417934541E-4	0.0	0.0	1.406145417934541E-4	0.0
22	1.8748605572460549E-4	0.0	0.0	1.406145417934541E-4	0.0
23	1.8748605572460549E-4	0.0	0.0	1.406145417934541E-4	0.0
24	1.8748605572460549E-4	0.0	0.0	3.281005975180596E-4	0.0
25	2.3435756965575684E-4	0.0	0.0	3.7497211144921097E-4	0.0
26	2.3435756965575684E-4	0.0	0.0	4.2184362538036233E-4	0.0
27	2.3435756965575684E-4	0.0	0.0	4.2184362538036233E-4	0.0
28	2.3435756965575684E-4	0.0	0.0	8.436872507607247E-4	0.0
29	2.812290835869082E-4	0.0	0.0	0.0014998884457968439	0.0
30	2.812290835869082E-4	0.0	0.0	0.002015475099039509	0.0
31	2.812290835869082E-4	0.0	0.0	0.003937207170216715	0.0
32	2.812290835869082E-4	0.0	0.0	0.006280782866774284	0.0
33	2.812290835869082E-4	0.0	0.0	0.009046202188712214	0.0
34	2.812290835869082E-4	0.0	0.0	0.012561565733548568	9.374302786230274E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1340	0.0	24.69121	1
GTATCAA	3190	0.0	24.389578	1
GTATACG	55	0.007661189	18.229324	1
ATCAACG	4320	0.0	17.971031	3
TATCAAC	4330	0.0	17.939207	2
TCAACGC	4415	0.0	17.62842	4
CAACGCA	4455	0.0	17.41951	5
AACGCAG	4635	0.0	16.864965	6
CCGATCG	75	0.0024492727	15.993683	9
ATACCGT	165	1.007902E-7	14.539711	6
ACGCAGA	5415	0.0	14.177225	7
TATACCG	100	0.0010180592	14.000706	5
ATTTGCG	130	3.3793585E-5	13.841335	16
CTATACC	220	1.2696546E-9	13.641529	4
CGCAGAG	5595	0.0	13.506727	8
ACCGTCG	105	0.0014762286	13.328068	8
AGAGTAC	5405	0.0	13.241762	11
GTATAGT	330	0.0	12.760526	1
CAGAGTA	5720	0.0	12.547491	10
GAGTACT	2545	0.0	12.490154	12
>>END_MODULE