FastQCFastQC Report
Mon 6 Feb 2017
SRR2077308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133492
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.040.257641
GTATCAA42050.036.9442441
ATCAACG57750.026.5815033
TCAACGC57800.026.4985584
TATCAAC58300.026.3932822
CAACGCA58550.026.0987115
AACGCAG62150.024.9179536
CGCTATA750.001935300923.1176411
ACGCAGA71350.021.3716097
CGCAGAG74500.020.367728
CGAATCG2303.4742698E-1019.48948737
AGAGTAC74750.019.47098511
GTACATG57950.018.9688341
GAGTACT49450.018.4129912
CAGAGTA80700.018.20549210
GTATTAG4000.018.2051451
GCAGAGT82850.018.0660659
TACATGG60150.017.9185852
AGTACTT51600.017.44526113
GACCGCG1804.2005213E-617.2495967
Produced by FastQC (version 0.11.5)