FastQCFastQC Report
Mon 6 Feb 2017
SRR2077307_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077307_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341246
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.2275284186283714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.10678074837073934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG200.001257890339.98799519
GTATCAA35150.024.0155091
GGTATCA14800.023.7088991
CGAATCG1602.0736479E-1017.50446937
TATCAAC48050.017.463282
ATCAACG48650.017.2768733
TCAACGC48450.017.2209634
CGCATAT700.001543659117.13734814
CAACGCA49450.016.8272155
CGTCGTA852.9733233E-416.46494110
AACGCAG51200.016.283136
TACCGAT2104.0017767E-1115.2325467
GTATTAC2900.015.2110691
GTATTAG2900.014.5196571
GTACCGA2351.4551915E-1114.4628186
GACCGTA850.005630837814.1125067
ATAAGGC3050.013.7790143
ACGCAGA59800.013.573657
GTATACG900.0080639113.3673041
CGCAGAG60950.013.2519418
Produced by FastQC (version 0.11.5)