FastQCFastQC Report
Mon 6 Feb 2017
SRR2077307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341246
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46350.039.4310531
GGTATCA20650.038.962371
TATCAAC64700.028.0760882
ATCAACG66250.027.261063
TCAACGC66850.026.9645294
CAACGCA68750.026.2691885
AACGCAG70850.025.4872846
ACGCAGA80550.022.1884467
CGCAGAG82700.021.5588198
GAGTACT55150.020.6373912
AGAGTAC83700.020.14975511
GTACATG61900.019.6089741
ATACCGT1801.8893661E-719.2554266
CAGAGTA89150.019.1879310
AGTACTT59500.019.18657113
GTACTTT59350.019.11557214
TACATGG64700.018.8066922
GCAGAGT93250.018.7133249
TGCGATA1301.1844632E-418.57124513
ACATGGG66950.017.397213
Produced by FastQC (version 0.11.5)