FastQCFastQC Report
Mon 6 Feb 2017
SRR2077306_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077306_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320682
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.19369306091916083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12650.026.0006031
GTATCAA30250.023.2708261
TATCAAC39750.017.3294772
ATCAACG40500.017.0974853
TCAACGC40550.017.0222764
CAACGCA41050.016.8109535
ACGGCGT750.002449235615.9938048
AACGCAG43350.015.9569096
GCGCATT800.003764606814.994513529
ACGCAGA50350.013.6193517
GTCTTAT3600.013.3702271
CGCAGAG51400.013.2244498
GTTCGAT1406.7391404E-512.86047635
TAGCCCT8000.012.7546814
TAAGGCG1100.002086865712.7289255
GGACCGT1100.002094992712.7223436
GTACATG57100.012.7145951
TACATGG57850.012.5997572
GTATAGC4900.012.278781
AGAGTAC50500.012.23288511
Produced by FastQC (version 0.11.5)