FastQCFastQC Report
Mon 6 Feb 2017
SRR2077306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320682
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.037.961381
GTATCAA43700.036.8892141
ATCAACG60900.026.2303773
TATCAAC61600.026.047632
TCAACGC61700.025.8902784
CAACGCA63400.025.1408585
AACGCAG67000.023.737266
GTACATG57050.021.1471311
ACGCAGA76400.020.7327617
CGCAGAG77200.020.3285888
GAGTACT52100.020.12734212
TACATGG59550.019.6699332
AGAGTAC76650.019.44115811
AGTACTT55500.019.39153513
GTACTTT57300.018.35543814
CAGAGTA83000.018.32743610
ACATGGG61550.018.2968483
GCAGAGT86200.017.9664219
TATACTG8200.016.9027615
GTATACG1452.6248497E-416.736241
Produced by FastQC (version 0.11.5)