FastQCFastQC Report
Mon 6 Feb 2017
SRR2077305_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077305_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144484
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.18489296259613033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30050.024.5613381
GGTATCA11450.023.2967261
GTACGGG659.1954635E-418.5134181
TATCAAC40600.018.297692
ATCAACG40800.018.0011753
TCAACGC40800.017.9973954
CAACGCA41050.017.9315075
AACGCAG42750.017.3515266
GCGATAC1005.6389315E-515.99520714
TAAGGCG1453.6890015E-715.1742375
GGTGCGA1201.587312E-514.99550511
ACGCAGA49250.014.858497
CGCAGAG50800.014.2870578
TACCGTT850.00562822414.1134177
AGAGTAC48300.013.95027111
CGTGCCG1151.8548466E-413.90887510
GCACCGT2453.092282E-1113.8733926
TATAGGG2056.8248482E-913.67484952
GTATAAA5500.013.4923541
GTACTAA2555.820766E-1113.3708011
Produced by FastQC (version 0.11.5)