FastQCFastQC Report
Mon 6 Feb 2017
SRR2077305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144484
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.040.0431631
GTATCAA40500.037.1689151
TATCAAC56500.026.3840312
TCAACGC56300.026.3539874
ATCAACG56500.026.1987273
CAACGCA57050.026.0682935
AACGCAG58650.025.2359666
ACGCAGA69550.021.0848927
CGCAGAG71850.020.3505218
CTCGTAT2950.019.8706839
GAGTACT48700.019.54459812
AGAGTAC72500.019.40746311
TATGCCG2850.019.3575743
CGATTCG900.005753827719.15637646
CAGAGTA76750.018.91647310
TATCTCG3150.018.6090536
AGTACTT51550.018.59784913
GTACTTT52150.018.44871514
GTACATG53200.018.3206081
GCAGAGT79850.018.1820839
Produced by FastQC (version 0.11.5)