FastQCFastQC Report
Mon 6 Feb 2017
SRR2077304_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077304_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927963
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.14113341386738232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.024.7058121
GTATCAA24550.022.1322731
ACCGGTA500.004474756320.00756634
TATGCGC605.4831686E-419.99199910
TTAGTCG751.134714E-418.67518239
TAGTCGG801.861114E-417.50661840
TATCAAC32300.016.6080322
ATCAACG33250.016.004393
TCAACGC33550.015.8579884
CAACGCA34500.015.418925
AACGCAG36300.014.8150076
ACCGTTA850.005601745614.12335435
CCTATAC2553.6379788E-1214.1215213
CGAAATC850.00563073214.11236613
GTATAAT4500.013.8120271
GTGCTAG3500.013.7483771
CGTATTA1050.001475618713.328691528
CTATGCG900.00822170913.3279999
CCGTGCG900.00822170913.3279999
GGTCGGT1551.2277145E-512.89806411
Produced by FastQC (version 0.11.5)