FastQCFastQC Report
Mon 6 Feb 2017
SRR2077304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927963
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32550.039.6219441
GGTATCA14300.036.8511771
TATCAAC45900.027.858242
TCAACGC46700.027.2311864
ATCAACG46800.027.1729983
CAACGCA46800.027.0235085
AACGCAG50000.025.5705326
ACGCAGA57500.022.0274667
CGCAGAG59250.021.1318058
GAGTACT39400.020.48668912
AGAGTAC60650.019.33749811
GTACTTT42100.019.08394814
GTACATG48200.018.9170111
AGTACTT42600.018.86680813
CAGAGTA65100.018.33310310
GCAGAGT67700.017.9856839
ACCGTCG1351.5804422E-417.8847398
TACATGG50700.017.497342
TACTTTT48850.016.72931515
ACATGGG52700.016.6351723
Produced by FastQC (version 0.11.5)