FastQCFastQC Report
Mon 6 Feb 2017
SRR2077303_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077303_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439279
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.22863313298724747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25950.10638389458524425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.10421112140103694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36200.023.480581
GGTATCA15400.020.0470981
TATCAAC47500.017.7025682
ATCAACG48900.016.8987463
TCAACGC49150.016.8514254
CAACGCA49450.016.7053075
AACGCAG51050.016.21366
GTATTAG3800.014.7715471
TATACTG5600.014.2870545
ACGCAGA58600.013.919947
GGACCGT1354.819543E-513.3285716
GTACATG64850.013.2616881
CGCAGAG60800.013.1531958
CTTAGAC3500.013.1484053
AGAGTAC57250.012.92114711
TAAGGCG1551.221803E-512.9044375
TACATGG64900.012.8947292
GAGTACT27650.012.79858212
CGGCACG1253.7515088E-412.79831322
TTATACC4700.012.7697744
Produced by FastQC (version 0.11.5)