FastQCFastQC Report
Mon 6 Feb 2017
SRR2077303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439279
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49150.039.17191
GGTATCA21050.036.585251
TATCAAC66300.028.6617052
TCAACGC66500.028.5743264
ATCAACG66800.028.3415993
CAACGCA68350.027.7991985
AACGCAG71650.026.7597036
ACGCAGA83050.022.8906867
CGCAGAG86200.022.1619958
GAGTACT57150.021.78421212
AGAGTAC84900.021.0413211
AGTACTT59500.020.92382813
GTACATG62400.020.347061
CTATTCG850.004137609620.28043763
CAGAGTA90550.020.1072110
GCAGAGT93900.019.720379
GTACTTT63850.019.38870814
TACATGG64200.019.2287042
ACATGGG66150.017.8220313
TACTTTT71100.017.4601615
Produced by FastQC (version 0.11.5)