FastQCFastQC Report
Mon 6 Feb 2017
SRR2077302_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077302_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294666
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.22931441874329425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.10498259877472363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23420.10206278386484134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.023.3093221
GTATCAA30250.023.1903041
TATACCG700.001541332917.1411865
TATCAAC42100.016.3525682
ATCAACG43000.015.863763
TCAACGC43000.015.8609924
CAACGCA43850.015.6883215
AACGCAG46150.015.0318666
GTCCTAA3900.013.8759571
GTGTAGC6950.013.5542911
TAGCCCT8300.013.4944254
TAGTACT4050.013.3337774
ACGCGGC1100.002095220412.7221497
ACGCAGA54100.012.6381647
GTATAAT4950.012.5522121
CTACACG2252.5533154E-812.4448594
GCCGACG1459.4162126E-512.4087968
ATAGCCC4200.012.3835243
CGCAGAG55650.012.32208258
TTACGGT1305.214028E-412.3081024
Produced by FastQC (version 0.11.5)