FastQCFastQC Report
Mon 6 Feb 2017
SRR2077302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294666
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.042.0958061
GTATCAA46350.038.7323071
TATCAAC64600.027.8334142
ATCAACG64400.027.7566453
TCAACGC64850.027.5100174
CAACGCA65850.027.0378675
AACGCAG69300.025.8412156
ACGCAGA81100.022.0816767
GAGTACT59700.021.72391312
CGCAGAG83000.021.485088
AGAGTAC83900.020.47349411
CAGAGTA88000.019.87237710
AGTACTT65250.019.77040713
GTACATG56250.019.4080261
GCAGAGT91750.019.097759
TACATGG56100.019.0203952
GTACTTT68250.018.7448614
TCGAACG1351.5833382E-417.88070753
ACATGGG57200.017.6238233
CGAATCG1602.9640369E-517.24211137
Produced by FastQC (version 0.11.5)