FastQCFastQC Report
Mon 6 Feb 2017
SRR2077301_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077301_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4845406
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70300.039.4547041
GGTATCA29800.032.1830061
TCAACGC88650.030.5484354
ATCAACG90250.030.1215343
CAACGCA89800.030.0419755
TATCAAC92700.029.6231582
AACGCAG94050.028.7577786
ACGCAGA107750.024.9732697
CGCAGAG111600.024.018758
AGAGTAC114200.021.78001811
CGCGTAA800.002901927321.5610165
CAGAGTA125850.020.33947610
GTACATG124300.020.2882371
GCAGAGT130750.020.0257689
TACATGG128750.019.2654742
GAGTACT58450.018.71015212
GTATACG2105.0496965E-818.0705531
ACATGGG131750.017.9365123
AGTACTT66050.016.66191313
GTATAGC14550.016.5971341
Produced by FastQC (version 0.11.5)