FastQCFastQC Report
Mon 6 Feb 2017
SRR2077301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4845406
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92150.042.179041
GGTATCA36400.037.5866241
TATCAAC128750.029.7057062
ATCAACG128900.029.6443333
TCAACGC129400.029.6310734
CAACGCA131300.029.0955585
AACGCAG135400.028.2620416
ACGCAGA157100.024.246327
CGCAGAG163200.023.251118
CGTACTA9500.022.51051729
GAGTACT110700.022.27797712
AGAGTAC163850.022.08240711
AGTACTT117650.021.02057513
CAGAGTA175650.020.91311810
GCAGAGT180450.020.5288569
GTACTTT122500.020.01939214
CGCGTAA900.00574479119.1621810
ACCGTAC11200.019.0937427
TATGCCG11100.018.95501943
GTACATG122600.018.8809491
Produced by FastQC (version 0.11.5)