FastQCFastQC Report
Mon 6 Feb 2017
SRR2077300_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077300_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241343
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67550.038.15021
GGTATCA27300.035.004081
TCAACGC85700.029.1044274
ATCAACG87000.028.6695333
CAACGCA87700.028.4013615
TATCAAC92150.027.3667742
AACGCAG91350.027.2287886
ACGCAGA108100.022.8501427
CGCAGAG112850.021.795878
AGAGTAC110800.021.48353811
GTACATG126750.020.9849531
CAGAGTA119100.020.3045110
TACATGG127950.019.8714142
GAGTACT54650.019.63279212
GCAGAGT128500.018.8460439
ACATGGG133650.018.2753583
AGTACTT61100.018.01197213
GTACTTT60450.017.52203814
GGACCGT4400.016.4652446
CATGGGG94100.015.8744934
Produced by FastQC (version 0.11.5)