FastQCFastQC Report
Mon 6 Feb 2017
SRR2077300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241343
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95250.042.074951
GGTATCA34250.036.6164861
ATCAACG129350.030.7425233
TATCAAC130400.030.2830262
TCAACGC130800.030.263584
CAACGCA133950.029.4996265
AACGCAG139550.028.3111576
ACGCAGA163100.024.1371887
GAGTACT108600.023.7574812
CGCAGAG166350.023.5558918
AGAGTAC163800.022.8688611
AGTACTT114950.022.50509813
TCGTATG15550.022.40369240
CTCGTAT14700.022.29127339
TATGCCG15750.021.900243
GTACTTT119900.021.60475514
CAGAGTA176650.021.45915810
GGCGAAT16350.021.3074932
GCAGAGT182000.021.0557799
GCGAATC16050.020.84612533
Produced by FastQC (version 0.11.5)