FastQCFastQC Report
Mon 6 Feb 2017
SRR2077299_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077299_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4936480
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80100.040.5275731
GGTATCA31850.031.411021
TCAACGC104250.030.6427974
ATCAACG104500.030.5364743
TATCAAC108350.029.9608522
CAACGCA107500.029.7484765
AACGCAG110850.028.7560836
ACGCAGA128400.024.6644347
CGCAGAG133200.023.4907288
AGAGTAC130950.022.44518511
GAGTACT63850.021.82866312
CAGAGTA144550.020.6914110
GCAGAGT148750.020.4086729
GTACATG150550.020.3940141
TACATGG150950.019.8143312
GTACTTT73550.018.7631614
AGTACTT75550.018.676513
GTATATA22450.018.2862241
ACATGGG157300.018.1810633
CATGGGG108250.016.5080344
Produced by FastQC (version 0.11.5)