FastQCFastQC Report
Mon 6 Feb 2017
SRR2077299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4936480
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116450.043.4381181
GGTATCA45000.035.7034231
TATCAAC158450.031.6842042
ATCAACG158850.031.5391653
TCAACGC161100.031.0942574
CAACGCA163800.030.4747145
AACGCAG169200.029.5183436
ACGCAGA193900.025.5779327
CGCAGAG199650.024.8794358
AGAGTAC197500.023.96092411
GAGTACT132300.023.62027412
CAGAGTA213550.022.41849710
GCAGAGT217450.022.4129669
AGTACTT140300.022.27343213
GTACTTT145450.021.22393414
TACTTTT160600.019.23602515
GTACATG143300.019.0721511
ACTTTTT170250.018.34844416
CTCGTAT10000.018.28068439
TACATGG144150.018.2406142
Produced by FastQC (version 0.11.5)