FastQCFastQC Report
Mon 6 Feb 2017
SRR2077298_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077298_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881993
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54800.040.85691
TCAACGC71950.030.4949284
ATCAACG72600.030.1743833
TATCAAC74900.029.5241572
CAACGCA74800.029.3791455
GGTATCA23200.029.2940581
AACGCAG78950.027.8348316
ACGCAGA91550.023.7778367
CGCAGAG94350.022.7431058
AGAGTAC90050.021.79866211
GAGTACT43550.021.54676212
CAGAGTA97500.020.62838410
GCAGAGT102700.019.9870119
GTACTTT47750.019.5799814
AGTACTT48900.019.40104713
GTACATG97500.019.2838231
TACATGG97650.018.724272
ACATGGG102650.017.5433623
GTATAGC7300.016.5404191
GTATATA10150.016.3146021
Produced by FastQC (version 0.11.5)