FastQCFastQC Report
Mon 6 Feb 2017
SRR2077298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881993
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73600.041.5435831
GGTATCA28750.038.094291
TCAACGC101950.029.7822274
ATCAACG102350.029.6377453
TATCAAC102900.029.3114572
CAACGCA104850.028.9250455
AACGCAG108850.027.9832126
ACGCAGA123300.024.5335717
CGCAGAG126350.023.8341038
AGAGTAC124600.023.05945611
CGTCGAA750.00199633822.99486440
GAGTACT81300.022.86758612
CTCGTAT14500.022.8362839
TCGTATG16250.022.71185140
TATGCCG15850.022.41455543
CAGAGTA132300.021.92594310
TCTCGTA14800.021.9072738
AATCTCG14900.021.7602436
ATGCCGT17000.021.50696244
AGTACTT86600.021.3884113
Produced by FastQC (version 0.11.5)