FastQCFastQC Report
Mon 6 Feb 2017
SRR2077297_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077297_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522122
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56900.041.8353651
GGTATCA22850.035.027451
TCAACGC78100.030.1259044
ATCAACG78950.029.7578623
TATCAAC79550.029.6635192
CAACGCA79800.029.440895
AACGCAG81850.028.6613736
ACGCAGA95950.024.305727
GAGTACT40800.023.8449112
CGCAGAG99950.023.1944778
AGAGTAC94700.023.16889211
CGGACTA750.001992400323.00253729
AGTACTT45150.021.9296113
CAGAGTA101650.021.89023610
GTACTTT44750.021.58640514
GCAGAGT104650.021.4934679
GTACATG110450.021.0211321
TACATGG114600.020.0190662
ACATGGG117250.018.8898683
TATAGCG950.00783826418.157385
Produced by FastQC (version 0.11.5)