FastQCFastQC Report
Mon 6 Feb 2017
SRR2077297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522122
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.11359482213786644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.042.3325461
GTATCAA74800.042.2908441
TATCAAC108450.028.977732
ATCAACG109900.028.5639763
TCAACGC110200.028.5118964
CAACGCA112850.027.718335
AACGCAG114700.027.3309236
ACGCAGA131400.023.7245717
CGCAGAG135050.022.8365358
GAGTACT84600.022.83339512
AGAGTAC132150.022.2134111
AGTACTT87900.022.17238613
GTACTTT88950.021.52285414
CAGAGTA139550.021.23323410
GCAGAGT145000.020.7920029
GTACATG104300.020.3961831
GCGCTTA850.00412516820.29102113
TACTTTT96850.019.7057815
TACATGG107250.019.448772
ACTTTTT101250.019.25846116
Produced by FastQC (version 0.11.5)