FastQCFastQC Report
Mon 6 Feb 2017
SRR2077296_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077296_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009270
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36800.040.9667851
GGTATCA14750.032.9780771
TCAACGC48050.030.2982374
TATCAAC49100.030.21242
CAACGCA48700.029.7521675
ATCAACG49450.029.4404473
AACGCAG51100.028.6248516
ACGCAGA60300.023.9142767
CGCAGAG62250.022.9983258
AGAGTAC61950.021.494811
GTACATG65900.020.7827661
CAGAGTA67000.020.38905110
TACATGG64800.020.0175572
GCAGAGT68800.020.0060379
GAGTACT31400.019.88598812
AGTACTT33950.019.00203313
ACATGGG68400.018.4092043
GTATATA9800.017.953221
GTACTTT35700.017.49206414
GTATTAC3950.017.4675161
Produced by FastQC (version 0.11.5)