FastQCFastQC Report
Mon 6 Feb 2017
SRR2077296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009270
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51100.043.407151
GGTATCA19550.036.4055521
TATCAAC68950.031.8691142
TCAACGC68550.031.8006944
ATCAACG68900.031.6415183
CAACGCA69600.031.320165
AACGCAG71400.030.383156
ACGCAGA83150.026.0052977
CGCTATC6900.025.49432633
CGCAGAG85150.025.1976838
TATCTCG7000.025.13012136
TACGCTA7250.024.7393231
GAGTACT58100.024.34053812
ACGCTAT7300.024.09737832
AGAGTAC86700.023.8701211
CTCGTAT7550.023.2994539
TATGCCG7550.023.2994543
CAGAGTA90600.023.07102410
AGTACTT62150.023.03188113
CGCTACG7900.022.70380828
Produced by FastQC (version 0.11.5)