FastQCFastQC Report
Mon 6 Feb 2017
SRR2077295_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077295_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824092
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81300.041.374631
GGTATCA31450.032.7997551
TCAACGC108950.030.5893294
TATCAAC109000.030.5436482
ATCAACG109550.030.3273143
CAACGCA111350.029.930025
AACGCAG116250.028.698136
ACGCAGA134500.024.6758927
CGCAGAG139050.023.668738
AGAGTAC139500.021.66411411
GTACATG166700.020.5510371
CAGAGTA151850.020.49244510
GAGTACT63350.020.36763412
GCAGAGT156950.020.2002289
TACATGG170200.019.6216662
ACATGGG171800.018.715993
AGTACTT71150.018.37722213
GTACTTT68750.018.3673514
GCGTACG1150.001110906518.000041
CATGGGG116050.017.0642344
Produced by FastQC (version 0.11.5)