FastQCFastQC Report
Mon 6 Feb 2017
SRR2077295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824092
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107850.042.3818931
GGTATCA41200.037.7360081
TATCAAC147350.030.8330362
TCAACGC149300.030.2411174
ATCAACG149350.030.2119433
CAACGCA150750.029.903175
AACGCAG157750.028.6389686
ACGCAGA180800.024.7756167
CGCAGAG188600.023.6489968
AGAGTAC187800.022.64554811
GAGTACT113600.022.0431512
CAGAGTA202600.021.36582610
GCAGAGT208600.020.9992989
AGTACTT119250.020.91198513
CGTACTA13300.020.48758729
GTACTTT123800.019.97624614
GTACATG160100.019.7240261
ACCGTAC13950.019.28571527
GACCGTA14400.018.92256426
TACATGG165050.018.5254362
Produced by FastQC (version 0.11.5)