FastQCFastQC Report
Mon 6 Feb 2017
SRR2077294_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077294_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658537
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71800.037.4297071
TCAACGC88200.029.3356744
ATCAACG89750.028.9827983
CAACGCA91150.028.3484025
TATCAAC94200.027.8700142
GGTATCA32600.027.6201691
AACGCAG96500.026.7052566
ACGCAGA111200.022.8957567
CGCAGAG115700.021.8859868
AGAGTAC113350.020.69643211
GTACATG124050.018.9666671
CAGAGTA126950.018.72362310
GCAGAGT131100.018.5519649
TACATGG122450.018.3129372
GAGTACT60850.018.1427112
GTATAGC14850.017.19131
GTACTTT64050.016.91382814
ACATGGG128400.016.8194873
AGTACTT65900.016.7520513
GTATTAG11700.015.9225031
Produced by FastQC (version 0.11.5)