FastQCFastQC Report
Mon 6 Feb 2017
SRR2077294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658537
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97900.041.0755231
GGTATCA41300.033.041511
ATCAACG132000.030.0455933
TATCAAC133350.029.9745832
TCAACGC133100.029.8966064
CAACGCA138700.028.713825
AACGCAG144500.027.5586286
ACGCAGA167700.023.4366747
TATGCCG12900.023.26251243
CGCAGAG173150.022.691878
AGAGTAC168950.021.8450311
GAGTACT115100.021.75646812
TCGTATG14900.020.8345140
CAGAGTA183300.020.58647210
CTCGTAT14550.020.38743239
GCAGAGT189750.020.2502569
AGTACTT123750.020.23571213
GGCGAAT14250.020.09047932
GTACTTT125550.019.72580714
GTACATG125000.019.484621
Produced by FastQC (version 0.11.5)