FastQCFastQC Report
Mon 6 Feb 2017
SRR2077293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803223
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75950.039.1549531
GGTATCA32100.031.3823221
TCAACGC97950.029.5152724
ATCAACG98950.029.1472593
TATCAAC101400.028.8172592
CAACGCA101150.028.6838445
AACGCAG105750.027.2403936
ACGCAGA124950.022.833717
CGCAGAG128750.021.9983238
AGAGTAC127150.020.64766911
GTACATG152050.019.8077471
CAGAGTA141150.019.08814810
GAGTACT59050.018.98782312
TACATGG154100.018.9845562
GCAGAGT144550.018.9732919
ACATGGG159100.017.7374173
AGTACTT66450.017.2367313
GTACTTT66450.016.77086614
ACCGTCG2603.3096512E-815.9221458
TAGCCCT27050.015.4321124
Produced by FastQC (version 0.11.5)