FastQCFastQC Report
Mon 6 Feb 2017
SRR2077293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803223
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109150.042.4245221
GGTATCA42100.038.7432331
TCAACGC149250.030.532494
ATCAACG149300.030.4832953
TATCAAC151250.030.0902842
CAACGCA152600.029.6581465
AACGCAG158450.028.6458996
ACGCAGA181750.024.7993957
CGCAGAG186650.023.9513518
GAGTACT124050.022.60508512
AGAGTAC188900.022.568311
AGTACTT131950.021.4869513
CAGAGTA201550.021.44275710
GCAGAGT207300.021.130849
CTCGTAT12300.021.03141439
TATGCCG14450.020.76652143
GTACTTT136800.020.22090514
GTACATG144450.019.593061
GACGTAG15050.019.4802626
TACATGG147750.018.8046172
Produced by FastQC (version 0.11.5)