FastQCFastQC Report
Mon 6 Feb 2017
SRR2077292_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077292_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076082
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74950.040.044531
GGTATCA30500.033.706371
TCAACGC94550.031.1960874
ATCAACG95750.030.769093
TATCAAC97000.030.443712
CAACGCA97400.030.3541055
AACGCAG102450.028.9252246
ACGCAGA119150.024.5236387
CGCAGAG120450.024.2016778
AGAGTAC122850.022.15657811
CAGAGTA130750.021.34530410
GAGTACT59500.020.46745912
GCAGAGT138350.020.3722259
GTACATG139000.019.830221
TACATGG135300.019.5310922
GTACTTT65350.018.74175614
AGTACTT68200.018.21060213
ACATGGG141400.018.0297873
GTATATG16550.016.050491
CATGGGG95300.015.8191844
Produced by FastQC (version 0.11.5)