FastQCFastQC Report
Mon 6 Feb 2017
SRR2077292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076082
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103300.043.88971
GGTATCA37000.041.2809451
TATCAAC140350.032.0573542
TCAACGC142050.031.5941224
ATCAACG142450.031.4877283
CAACGCA144750.031.0506275
AACGCAG148550.030.2747546
ACGCAGA171000.026.177287
CGCAGAG175700.025.3656868
GAGTACT114900.024.97563612
AGAGTAC176600.024.15973911
AGTACTT121400.023.49633213
CAGAGTA185400.023.36647410
GCAGAGT191350.022.85629
GTACTTT125850.022.50107214
GTACATG133550.020.5708541
CTCGTAT12700.020.3690539
TACTTTT138500.020.28602615
ACTTTTT143400.019.92982516
TACATGG134500.019.9117072
Produced by FastQC (version 0.11.5)