FastQCFastQC Report
Mon 6 Feb 2017
SRR2077291_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077291_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904655
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53700.038.0955541
ATCAACG66700.030.049983
TCAACGC67100.029.8708444
CAACGCA69550.028.86825
TATCAAC70550.028.654612
AACGCAG72150.027.7322756
GGTATCA24200.024.8043441
ACGCAGA83800.023.7122277
CGCAGAG87200.022.7079628
AGAGTAC83650.021.11505911
GTATAGC14600.020.3207361
GAGTACT45700.019.47614112
GTACATG92000.019.3863751
CAGAGTA94950.019.2195610
GCAGAGT100350.018.6322219
ATACCGT3001.8189894E-1218.3989326
TACATGG93850.018.3058092
AGTACTT50200.017.9364413
GTACTTT51000.017.45304514
ACATGGG97350.017.1869833
Produced by FastQC (version 0.11.5)