FastQCFastQC Report
Mon 6 Feb 2017
SRR2077291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904655
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67600.040.5308271
TCAACGC90400.030.1516974
GGTATCA29350.030.1363581
ATCAACG90900.030.0657143
TATCAAC91550.029.701292
CAACGCA91650.029.6624155
AACGCAG97050.028.0069276
ACGCAGA112650.023.9724127
CGCAGAG117550.022.9484238
GAGTACT77850.022.41899512
AGAGTAC116600.022.21598611
AGTACTT84950.020.50464413
CAGAGTA128500.020.48073810
GTACTTT85850.020.12897514
GCAGAGT133150.019.8691089
GTACATG91250.018.5533431
TATGCCG15650.018.0727343
TACATGG91800.017.7647152
TACTTTT99250.017.63089215
ACTTTTT101450.017.4526816
Produced by FastQC (version 0.11.5)