FastQCFastQC Report
Mon 6 Feb 2017
SRR2077290_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077290_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713524
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.039.617381
GTATCAA80850.033.6412431
ATCAACG101250.026.507943
TATCAAC102950.026.5047662
TCAACGC101300.026.3930474
CAACGCA104800.025.5112555
AACGCAG107350.024.824916
ACGCAGA124300.021.3030267
CGCAGAG126450.020.7786188
GTACATG102250.019.916571
AGAGTAC125150.019.75622611
GTGGTAT18950.019.3073441
TACATGG103000.018.9227852
GAGTACT80000.018.87990412
CAGAGTA134200.018.80911410
GCAGAGT137800.018.442859
AGTACTT85150.017.78067613
ACATGGG108700.017.4234523
GTACTTT85550.017.37479814
TGGTATC21450.015.4389512
Produced by FastQC (version 0.11.5)