##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077290_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3713524 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.999023030415316 31.0 31.0 34.0 26.0 34.0 2 30.04128019638489 31.0 31.0 34.0 26.0 34.0 3 30.14972382028499 31.0 31.0 34.0 26.0 34.0 4 33.55936221228138 37.0 35.0 37.0 28.0 37.0 5 33.58822617007457 37.0 35.0 37.0 28.0 37.0 6 33.61275893194712 37.0 35.0 37.0 28.0 37.0 7 33.524507179703164 37.0 35.0 37.0 28.0 37.0 8 33.424135672746424 37.0 35.0 37.0 27.0 37.0 9 34.844897191993375 39.0 35.0 39.0 27.0 39.0 10 34.76122841807405 39.0 35.0 39.0 27.0 39.0 11 34.78923092997379 39.0 35.0 39.0 27.0 39.0 12 34.70893334740801 39.0 35.0 39.0 27.0 39.0 13 34.676574057418236 39.0 35.0 39.0 27.0 39.0 14 35.80635805773707 40.0 36.0 41.0 26.0 41.0 15 35.82966853048479 40.0 36.0 41.0 26.0 41.0 16 35.81236098110582 40.0 36.0 41.0 26.0 41.0 17 35.74647935492002 40.0 36.0 41.0 26.0 41.0 18 35.706393441916624 40.0 36.0 41.0 25.0 41.0 19 35.800815613417335 40.0 36.0 41.0 26.0 41.0 20 35.788990726867524 40.0 36.0 41.0 26.0 41.0 21 35.76732693797051 40.0 36.0 41.0 26.0 41.0 22 35.705950466457196 40.0 36.0 41.0 25.0 41.0 23 35.66566501253257 40.0 36.0 41.0 25.0 41.0 24 35.64519146772715 40.0 36.0 41.0 25.0 41.0 25 35.67114040463991 40.0 36.0 41.0 25.0 41.0 26 35.62105374840717 40.0 36.0 41.0 25.0 41.0 27 35.61696383273677 40.0 36.0 41.0 25.0 41.0 28 35.581799659837934 40.0 36.0 41.0 25.0 41.0 29 35.55341880111721 40.0 36.0 41.0 25.0 41.0 30 35.47232817130036 40.0 36.0 41.0 25.0 41.0 31 35.45012015541033 40.0 36.0 41.0 25.0 41.0 32 35.38570775360547 40.0 35.0 41.0 25.0 41.0 33 35.272575591271256 40.0 35.0 41.0 24.0 41.0 34 35.23640105732453 40.0 35.0 41.0 24.0 41.0 35 35.18896444455455 40.0 35.0 41.0 24.0 41.0 36 35.11370143292463 40.0 35.0 41.0 24.0 41.0 37 35.037753896299044 40.0 35.0 41.0 24.0 41.0 38 34.955912766418095 40.0 35.0 41.0 23.0 41.0 39 34.90702604857273 40.0 35.0 41.0 23.0 41.0 40 34.86567933854743 40.0 35.0 41.0 23.0 41.0 41 34.779979609664565 40.0 35.0 41.0 22.0 41.0 42 34.68972544677239 39.0 35.0 41.0 22.0 41.0 43 34.592810225543175 39.0 35.0 41.0 21.0 41.0 44 34.48901609360812 39.0 34.0 41.0 19.0 41.0 45 34.3872442994848 39.0 34.0 41.0 18.0 41.0 46 34.28499802344081 39.0 34.0 41.0 18.0 41.0 47 34.16803230570208 39.0 34.0 41.0 15.0 41.0 48 34.054323602055625 39.0 34.0 41.0 15.0 41.0 49 33.94927702096445 39.0 33.0 41.0 13.0 41.0 50 33.822522757359316 39.0 33.0 41.0 9.0 41.0 51 33.722620346603385 39.0 33.0 41.0 9.0 41.0 52 33.62019149465575 39.0 33.0 41.0 8.0 41.0 53 33.539636205394125 38.0 33.0 40.0 8.0 41.0 54 33.541302277836365 39.0 33.0 41.0 7.0 41.0 55 33.45126704445696 38.0 33.0 41.0 7.0 41.0 56 33.318649616913746 38.0 33.0 40.0 2.0 41.0 57 33.22736166509224 38.0 33.0 40.0 2.0 41.0 58 33.13325832820792 38.0 33.0 40.0 2.0 41.0 59 33.02030901106335 38.0 33.0 40.0 2.0 41.0 60 33.00088918235078 38.0 33.0 40.0 2.0 41.0 61 32.89400580149744 38.0 33.0 40.0 2.0 41.0 62 32.7453192708597 37.0 32.0 40.0 2.0 41.0 63 32.559943600741505 37.0 32.0 40.0 2.0 41.0 64 32.418592689854705 37.0 32.0 40.0 2.0 41.0 65 32.272174355140834 37.0 32.0 40.0 2.0 41.0 66 32.10831759805511 36.0 32.0 40.0 2.0 41.0 67 31.941984756258474 36.0 32.0 39.0 2.0 41.0 68 31.75373526601686 36.0 32.0 39.0 2.0 41.0 69 31.78032860431224 36.0 32.0 39.0 2.0 41.0 70 31.68201551949038 36.0 32.0 39.0 2.0 41.0 71 31.522062332167504 35.0 32.0 39.0 2.0 41.0 72 31.323476837634548 35.0 32.0 39.0 2.0 41.0 73 31.119111388535526 35.0 32.0 39.0 2.0 40.0 74 30.90212908277959 35.0 32.0 38.0 2.0 40.0 75 30.678463637235144 35.0 31.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.762680889719384 1101 2 0.8164165210962793 1101 3 0.8560139714452077 1101 4 0.8386736868523883 1101 5 0.8246302562429122 1101 6 0.7728078665454134 1101 7 0.807832015821063 1101 8 0.8938957063283794 1101 9 0.9603510048394241 1101 10 1.0161272010136457 1101 11 0.9096287856056762 1101 12 0.9554017684251122 1101 13 0.9159765626894227 1101 14 1.0515778556860624 1101 15 1.0406697224914154 1101 16 1.1387760634461372 1101 17 1.0671891170077146 1101 18 1.0738622245370593 1101 19 1.2010755332860512 1101 20 1.103185177969209 1101 21 1.1232263871889288 1101 22 1.1664201248975345 1101 23 1.1306383493881427 1101 24 1.094976612003812 1101 25 1.1204906315600667 1101 26 1.0794905243664772 1101 27 1.1278612463982753 1101 28 1.1632393197341244 1101 29 1.1675936913219616 1101 30 1.2349868097066903 1101 31 1.1988716239099517 1101 32 1.173111264806174 1101 33 1.184103590809194 1101 34 1.1947518431034112 1101 35 1.199621655384803 1101 36 1.2725889927550398 1101 37 1.236265785607607 1101 38 1.071718648391915 1101 39 1.2678814606831281 1101 40 1.1853248655896493 1101 41 1.2222183098947355 1101 42 1.1253958105107102 1101 43 1.1492956067055005 1101 44 1.222632837209325 1101 45 1.1566555058344292 1101 46 1.1599290014704238 1101 47 1.189785780742902 1101 48 1.2095407357732455 1101 49 1.2124628213623438 1101 50 1.1863142285357355 1101 51 1.1065119253891922 1101 52 1.1517771365263982 1101 53 1.1710503979832154 1101 54 1.1564341127433053 1101 55 1.0514958649112742 1101 56 1.0581110442402135 1101 57 1.0295380177102942 1101 58 0.9659197693709487 1101 59 0.9601394218654988 1101 60 1.036311291675176 1101 61 1.0747165090398951 1101 62 1.0471986545733998 1101 63 1.1176491882455366 1101 64 1.066540046009365 1101 65 1.0771279691029676 1101 66 1.0837205272781816 1101 67 0.9939935256698433 1101 68 1.0037809856968707 1101 69 1.052845507767799 1101 70 1.1330606535978163 1101 71 1.0506425699595638 1101 72 1.0347576571583623 1101 73 1.0933473139220347 1101 74 1.1628069461867057 1101 75 1.1448893525123651 1102 1 0.9149898251240778 1102 2 0.9042110229776519 1102 3 0.8773131010706834 1102 4 0.8757964779748448 1102 5 0.9141185727226713 1102 6 0.7804970218196701 1102 7 0.8826804135522366 1102 8 0.920202774518728 1102 9 1.1127947905802102 1102 10 1.101506702165409 1102 11 1.0525118095636614 1102 12 1.021949193273727 1102 13 1.0535040863145468 1102 14 1.1683427471197447 1102 15 1.158837493006999 1102 16 1.2351424004463851 1102 17 1.2773536017487572 1102 18 1.2510940792718515 1102 19 1.2046337672149008 1102 20 1.1801447185125156 1102 21 1.2503370399661975 1102 22 1.289315570331965 1102 23 1.2275003112844871 1102 24 1.2902548175033814 1102 25 1.1700005603924808 1102 26 1.248025748435829 1102 27 1.2856567210896088 1102 28 1.231429704451429 1102 29 1.2477329047833265 1102 30 1.2325022603246083 1102 31 1.23936933962149 1102 32 1.2863401372250536 1102 33 1.3361947692377854 1102 34 1.3373482148126428 1102 35 1.3396027291492132 1102 36 1.3166176456382956 1102 37 1.369836731403943 1102 38 1.3748531655042981 1102 39 1.3336651208152688 1102 40 1.3557372135971661 1102 41 1.304303397371406 1102 42 1.3396998557304016 1102 43 1.343463393192593 1102 44 1.3920847168741801 1102 45 1.2638622490046174 1102 46 1.2525319795756715 1102 47 1.2862257681689684 1102 48 1.3033061128859273 1102 49 1.3098023313129517 1102 50 1.2926025737642135 1102 51 1.3257341783444687 1102 52 1.3282270045073759 1102 53 1.3554178137861541 1102 54 1.4056316534216435 1102 55 1.3699614314315127 1102 56 1.3614043689489321 1102 57 1.3654973208589283 1102 58 1.3294854590895824 1102 59 1.3698608787863478 1102 60 1.3629033060669542 1102 61 1.3915573377287913 1102 62 1.3845494389558795 1102 63 1.3216399596184942 1102 64 1.3172415581480692 1102 65 1.341850975985956 1102 66 1.3571886601291752 1102 67 1.3839665410830762 1102 68 1.361165847467106 1102 69 1.3316019154274414 1102 70 1.2887077065281147 1102 71 1.3121696420266318 1102 72 1.3458996344163943 1102 73 1.3419157587255945 1102 74 1.3426088704628825 1102 75 1.3601888944931098 1103 1 0.6239547270299362 1103 2 0.6823512286070397 1103 3 0.7076403236214404 1103 4 0.7301969676674389 1103 5 0.6290318909087347 1103 6 0.7277808879011971 1103 7 0.705162179765523 1103 8 0.7505113223519402 1103 9 0.8807733561544069 1103 10 0.8995961748121388 1103 11 0.9751657981720854 1103 12 0.8557665130367695 1103 13 0.9254546605621101 1103 14 0.9190202243729004 1103 15 0.93652691170454 1103 16 1.0218179894781017 1103 17 1.0681749780754402 1103 18 1.0819919181920383 1103 19 1.0562662487736176 1103 20 1.020653812062477 1103 21 1.0156056965277642 1103 22 1.0426132142051046 1103 23 1.090739129797356 1103 24 1.0124782501239622 1103 25 0.9555733036047371 1103 26 1.0779421272006005 1103 27 1.07774745946719 1103 28 0.9722770178858156 1103 29 0.9073737277158784 1103 30 0.9427002123305854 1103 31 0.9305714726892234 1103 32 0.9676317014401405 1103 33 0.9591174662819952 1103 34 1.0128853723131357 1103 35 1.0329654232884593 1103 36 1.0312243872113314 1103 37 0.9773429303726786 1103 38 0.9861072765358685 1103 39 1.0259607623436935 1103 40 1.0615419383007065 1103 41 1.08757655661487 1103 42 1.050139541580151 1103 43 1.0474566702942738 1103 44 0.9051144822989841 1103 45 0.903534092717976 1103 46 0.9308272891091107 1103 47 0.9528026924429867 1103 48 0.9424669955775755 1103 49 0.9265514779821089 1103 50 0.9811601593307344 1103 51 0.9972682026423456 1103 52 0.9254486215304212 1103 53 0.911949814989633 1103 54 0.9151889588673967 1103 55 0.925811805444603 1103 56 0.9243941912278686 1103 57 0.9460792458073115 1103 58 0.9107199616677306 1103 59 0.885833468450933 1103 60 0.9153880809847337 1103 61 0.925699390080247 1103 62 0.886177770577369 1103 63 0.9998258145574397 1103 64 0.9775790517934766 1103 65 0.9292901534684006 1103 66 0.941531984436395 1103 67 0.9231668481784325 1103 68 0.9491398481103488 1103 69 1.0094303570222607 1103 70 0.9804119820009518 1103 71 0.927242732796131 1103 72 0.898445620908344 1103 73 0.9278096033311947 1103 74 0.9483866205744143 1103 75 0.9607906353917777 1104 1 0.5933797176426552 1104 2 0.609182784036097 1104 3 0.6170977588157953 1104 4 0.7074192143879046 1104 5 0.6530598563207803 1104 6 0.5572045021581076 1104 7 0.6077404416285788 1104 8 0.6479734157757662 1104 9 0.6474391208403247 1104 10 0.7842468009204069 1104 11 0.64983263320849 1104 12 0.6601395161516095 1104 13 0.6830533472781397 1104 14 0.7534167591633008 1104 15 0.7922416947013389 1104 16 0.7603919980008129 1104 17 0.7727780530948465 1104 18 0.8361655167477977 1104 19 0.7538279275535515 1104 20 0.7123228840111437 1104 21 0.8240147962440716 1104 22 0.7829385018701487 1104 23 0.7839628824916005 1104 24 0.8583828900465633 1104 25 0.8218186116345052 1104 26 0.8790013198543392 1104 27 0.8749215871849927 1104 28 0.7695613542885553 1104 29 0.7966584970693802 1104 30 0.8829960352911357 1104 31 0.8822386003682752 1104 32 0.8489533504480278 1104 33 1.0017127722405448 1104 34 0.9819341701398301 1104 35 0.9510549478844723 1104 36 0.9489884971968578 1104 37 0.8841070176416608 1104 38 0.9593609081776222 1104 39 0.9362888762150874 1104 40 0.9049758191029511 1104 41 0.8964687171327057 1104 42 0.9064577162918184 1104 43 0.908914999451774 1104 44 0.8856943690636498 1104 45 0.9167583665566639 1104 46 0.9179858893953394 1104 47 1.0118110080373697 1104 48 0.9062259435606848 1104 49 0.9863748501072962 1104 50 0.8825576949856995 1104 51 0.9507970519955293 1104 52 0.9786238944297025 1104 53 0.9526650589988108 1104 54 0.9457245817619437 1104 55 0.8829662499380433 1104 56 0.9979116021288732 1104 57 0.9275113373469992 1104 58 0.912737625791479 1104 59 0.8920688279670941 1104 60 0.9651212283095916 1104 61 0.9538373477018496 1104 62 0.9665336988625484 1104 63 0.9196832882363069 1104 64 0.9098483485023863 1104 65 0.9194796095138713 1104 66 0.9675895067554094 1104 67 0.9274245647907762 1104 68 0.923687053773989 1104 69 0.9625761318103656 1104 70 0.9433646928205732 1104 71 0.9427984598393877 1104 72 1.060305966226668 1104 73 1.0376071520815877 1104 74 1.020534256783531 1104 75 1.047797636098661 1105 1 0.549169322973988 1105 2 0.5421596189786797 1105 3 0.5886669642972038 1105 4 0.49910266270424586 1105 5 0.48532222688513116 1105 6 0.5403180788317385 1105 7 0.5789856272731839 1105 8 0.6059755189057014 1105 9 0.6589424631323766 1105 10 0.7485401722325875 1105 11 0.6654490110776408 1105 12 0.6542827842215502 1105 13 0.5842791095989313 1105 14 0.7064805906883009 1105 15 0.8283309832870103 1105 16 0.7291340378837106 1105 17 0.7256622635405634 1105 18 0.8232231639477519 1105 19 0.7749327981880896 1105 20 0.8104391916908824 1105 21 0.7889068832176847 1105 22 0.8532079271082225 1105 23 0.8247715793422685 1105 24 0.8627584340144878 1105 25 0.805812937777894 1105 26 0.8779001118307974 1105 27 0.8511512418275728 1105 28 0.8139743411159159 1105 29 0.815846175892851 1105 30 0.8039972170255609 1105 31 0.769595445877556 1105 32 0.7823282869585171 1105 33 0.8761517674344859 1105 34 0.8589264008103044 1105 35 0.8958389814528758 1105 36 0.7867799427668842 1105 37 0.7796855315149145 1105 38 0.838369999620383 1105 39 0.8222428906664518 1105 40 0.8737801361344708 1105 41 0.8451263765838988 1105 42 0.7934451740824571 1105 43 0.7914252662480976 1105 44 0.8018284895393037 1105 45 0.8113792416526948 1105 46 0.8471111762147885 1105 47 0.8731855208590602 1105 48 0.9079352471030759 1105 49 0.9099549183569238 1105 50 0.9547847666580154 1105 51 1.017404312898499 1105 52 0.9317900110666386 1105 53 0.895687912670482 1105 54 0.8989495298855843 1105 55 0.9792543787394408 1105 56 0.9442254457774979 1105 57 0.9479567465767786 1105 58 0.9105953812107401 1105 59 0.8910536338742361 1105 60 0.8203463831729536 1105 61 0.8902643111548656 1105 62 0.8971061636848887 1105 63 1.0320555116070835 1105 64 0.9385115975829024 1105 65 0.9784163803416277 1105 66 1.049034170701276 1105 67 1.0345461736851789 1105 68 0.95123084558454 1105 69 0.9166355043936179 1105 70 0.9538128330564604 1105 71 0.9503233387945613 1105 72 0.9116775310931651 1105 73 0.8943155367808053 1105 74 0.9356354443883355 1105 75 0.8990490494581316 1106 1 0.3167789273290005 1106 2 0.2872637031903764 1106 3 0.36639613703950147 1106 4 0.3177725511904086 1106 5 0.2517000407695065 1106 6 0.2969818291962767 1106 7 0.22138449408149796 1106 8 0.23316934190005156 1106 9 0.3883887823424743 1106 10 0.4082910367078654 1106 11 0.34732488770417547 1106 12 0.28767166616686524 1106 13 0.322631260024572 1106 14 0.3184027115343042 1106 15 0.42969331299579494 1106 16 0.29380234323443233 1106 17 0.2805887310654569 1106 18 0.3486547072270625 1106 19 0.41319884826516784 1106 20 0.4324253323121994 1106 21 0.4787970358374949 1106 22 0.45528822262683377 1106 23 0.475477741072055 1106 24 0.5403334593585427 1106 25 0.4335844663368249 1106 26 0.5307879452960549 1106 27 0.5206272795450815 1106 28 0.40770984639997465 1106 29 0.5490059198137516 1106 30 0.6014628116040157 1106 31 0.500663784557446 1106 32 0.5335788250856979 1106 33 0.5536281939922745 1106 34 0.5235544334173383 1106 35 0.5105204795989451 1106 36 0.5509094265814056 1106 37 0.5215830721001211 1106 38 0.5133842339522019 1106 39 0.6342608328681152 1106 40 0.5653020292311695 1106 41 0.577465389762807 1106 42 0.4693856815190287 1106 43 0.6000894610015308 1106 44 0.5707865364501075 1106 45 0.621187737812896 1106 46 0.6124349551868633 1106 47 0.6644532790559481 1106 48 0.4365136192510022 1106 49 0.5004380524096135 1106 50 0.5053228938365635 1106 51 0.4415318525116021 1106 52 0.4669453388728826 1106 53 0.41072908807910835 1106 54 0.4590461844704308 1106 55 0.4537145156667677 1106 56 0.5258749618102172 1106 57 0.460905128029097 1106 58 0.5079498785652348 1106 59 0.4673163232602846 1106 60 0.4041150787526746 1106 61 0.4881270783089704 1106 62 0.5449321298835628 1106 63 0.5368424132443792 1106 64 0.6254896142197168 1106 65 0.5973042971298028 1106 66 0.6417198072189336 1106 67 0.6003879434639572 1106 68 0.6133786326667092 1106 69 0.5928801404807729 1106 70 0.566029361545958 1106 71 0.548583278524319 1106 72 0.5566052484618282 1106 73 0.622768924551778 1106 74 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138589.0 3 22124.0 4 15279.0 5 11266.0 6 9207.0 7 8309.0 8 7777.0 9 8112.0 10 8428.0 11 9031.0 12 9836.0 13 10351.0 14 10762.0 15 11461.0 16 12304.0 17 13173.0 18 13734.0 19 15181.0 20 17030.0 21 19881.0 22 25004.0 23 30444.0 24 33157.0 25 34213.0 26 35763.0 27 38125.0 28 44537.0 29 53430.0 30 60239.0 31 66409.0 32 80148.0 33 99886.0 34 130580.0 35 174466.0 36 242697.0 37 375388.0 38 622377.0 39 980265.0 40 214561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01451435585801 18.22509391840396 13.891290712026278 23.86910101371175 2 15.377663119794823 19.802211384358532 40.109760332699885 24.710365163146758 3 19.01015988725197 24.43487801044057 29.43708761942202 27.11787448288544 4 12.721829898610155 16.355655999989214 37.71119738185231 33.21131671954832 5 13.846099493143656 37.48627949537669 34.39338783598718 14.274233175492467 6 32.114003953707325 36.81903430752423 17.288221597422865 13.778740141345578 7 28.07731132675753 32.464884959417105 21.19415403596488 18.263649677860485 8 25.95757573633805 35.5024285603768 19.607980029171298 18.932015674113856 9 25.969297066691972 16.668261817981822 19.59145973060725 37.77098138471896 10 14.411491447684504 29.63078995344905 33.49840687140171 22.45931172746474 11 34.388227581890504 23.758432551854618 23.262180397621936 18.59115946863294 12 23.16512035854753 25.53162572017598 30.01816198549468 21.285091935781807 13 27.539820154255985 22.68580288817992 25.895446085249397 23.8789308723147 14 21.52997853647801 22.174936065243926 27.404262388994105 28.890823009283956 15 23.309578071053284 29.52183938918584 24.15941725313229 23.009165286628583 16 22.80037031633569 29.064600676180586 25.632055177457623 22.502973830026097 17 21.77688402754915 28.69579965254831 26.869561630073147 22.657754689829392 18 21.711490783295204 28.175843376011628 28.056531100059185 22.056134740633986 19 22.605945730301226 27.745806313437356 28.307778789895284 21.340469166366134 20 22.80158850871544 27.157339598482473 28.270298899823416 21.770772992978674 21 23.0911409434348 27.028853036637944 28.097012530187293 21.78299348973996 22 22.703853632619815 27.00533720971 28.040092450504726 22.250716707165452 23 22.44601079662684 27.04532635748849 27.88923598476399 22.61942686112068 24 22.130275436705276 27.064602093790395 28.273155581135118 22.531966888369208 25 22.545290722533878 26.9354997524876 28.12274996391849 22.396459561060027 26 22.31641961093071 27.072502139603284 28.114319684377158 22.496758565088847 27 22.356327172316753 27.294006631842123 27.982764332213637 22.366901863627486 28 22.319402466487475 27.232819670609864 28.051978811817303 22.395799051085362 29 22.37709585898487 27.288405680984045 28.063070609267655 22.271427850763427 30 22.559265689182467 27.25153511918956 27.954625642397886 22.234573549230095 31 22.553593953840195 26.95322405385778 28.024887319703435 22.468294672598592 32 22.311254200037926 27.31159540886663 27.91096310709884 22.466187283996604 33 22.37528730050824 27.102635071703734 27.944196259967846 22.577881367820186 34 22.5860943689093 27.205736935529085 28.020064920681133 22.18810377488048 35 22.709333917486084 27.209935920349476 27.818602225697525 22.262127936466914 36 22.583768509898068 27.107401585169473 27.924994867447968 22.38383503748449 37 22.6843087437238 26.86138177862012 27.9158158089013 22.538493668754775 38 22.446975277163954 26.988929630439063 28.02241594694425 22.541679145452733 39 22.530208259437273 27.419256951129746 27.767996899130292 22.282537890302688 40 22.782165969427606 27.114941241701594 27.88457984207906 22.218312946791734 41 22.598409582970802 27.11090427146355 27.891031477846802 22.399654667718853 42 22.601946380990864 27.182120482565143 27.671887446455358 22.544045689988636 43 22.421524663677133 27.026713440799433 27.98617498961829 22.56558690590515 44 22.536752079567027 27.362281039458423 27.6379739069745 22.462992974000052 45 22.692687132591665 27.316768266117275 27.499536671974816 22.491007929316243 46 22.777700568455508 27.15676445864496 27.362773922558898 22.702761050340627 47 22.618789377917935 27.314461071575806 27.398343186581453 22.66840636392481 48 22.512906864111752 27.45647399775289 27.397151947844094 22.633467190291263 49 22.670501917850515 27.32789211345211 27.251129508824594 22.750476459872782 50 22.644219935148087 27.536099682487418 27.333339630508735 22.486340751855753 51 22.58521887629386 27.70419506486138 27.0381043919932 22.672481666851557 52 22.75925851461659 27.601895678320844 27.0202437956098 22.618602011452765 53 22.712633603925497 27.563297005820296 27.12004863696944 22.60402075328476 54 22.646073105756265 27.425720349249122 27.304391838479024 22.623814706515585 55 22.55264372728408 27.36100510012878 27.23640903232456 22.84994214026258 56 22.540990574401025 27.828954807481864 27.13183775566738 22.498216862449727 57 22.719624857001016 27.51659327850806 27.037195924798723 22.7265859396922 58 22.602196271897274 27.457069064627827 27.2351209190985 22.705613744376404 59 22.650382383218865 27.652431009343786 27.105186020110544 22.592000587326805 60 22.52357592963579 27.60099092052068 27.26715716044804 22.608275989395487 61 22.778284106405838 27.328764590833245 27.35100286803607 22.541948434724855 62 22.616740746858984 27.550813559495886 27.355821645020246 22.476624048624892 63 22.543200450956338 27.33463854380842 27.26876609015671 22.85339491507853 64 22.569763189112667 27.43316540053524 27.308896029669178 22.688175380682914 65 22.538786452786024 27.354178731538216 27.25736277769453 22.84967203798123 66 22.48816843996131 27.56521391805997 27.17963568294873 22.766981959029987 67 22.58852301699669 27.36163280510832 27.202746896042562 22.847097281852427 68 22.648660872831762 27.367854902972518 27.252568311083813 22.730915913111907 69 22.620511220801145 27.46238594892973 27.058316216003252 22.85878661426587 70 22.512985858119162 27.26362256796002 27.28264897202093 22.94074260189989 71 22.583036505324824 27.255085521278914 27.160255280633017 23.00162269276324 72 22.342917698985847 27.442612678521165 27.20465191964817 23.009817702844813 73 22.639507504065186 27.269474437498687 27.138384687162866 22.952633371273265 74 22.549873298036854 27.49889973963428 27.047786243763728 22.903440718565136 75 22.604169337675327 27.499652487715608 27.04460708169305 22.85157109291601 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3282.0 1 2667.0 2 2349.5 3 2549.0 4 2451.0 5 2148.0 6 1732.5 7 1733.0 8 1846.0 9 2399.0 10 3683.0 11 4466.5 12 4519.0 13 4442.5 14 4600.0 15 5185.5 16 5537.0 17 6133.5 18 7273.0 19 9010.5 20 10205.0 21 11954.5 22 16022.5 23 21198.0 24 24055.0 25 28013.5 26 37012.5 27 46597.0 28 51141.0 29 56341.0 30 66154.0 31 76475.0 32 82183.0 33 87173.5 34 98180.0 35 109698.0 36 115200.0 37 119997.0 38 129063.0 39 137974.0 40 142616.0 41 147385.5 42 155792.5 43 162665.0 44 165900.0 45 168775.0 46 174271.0 47 177718.0 48 178544.0 49 177724.5 50 174743.5 51 169786.5 52 166991.0 53 164186.5 54 156526.5 55 144719.5 56 137768.0 57 130825.0 58 115490.0 59 97296.0 60 87494.0 61 78328.0 62 61480.5 63 45996.0 64 38193.0 65 32597.0 66 22674.5 67 15625.5 68 12903.0 69 10892.0 70 7452.5 71 5054.5 72 4085.0 73 3661.5 74 2919.0 75 2048.0 76 1496.0 77 1215.5 78 727.0 79 415.0 80 311.0 81 247.0 82 145.0 83 78.5 84 50.0 85 42.0 86 27.5 87 22.5 88 24.0 89 21.0 90 15.0 91 10.5 92 9.0 93 11.0 94 9.5 95 6.5 96 7.0 97 6.0 98 7.0 99 10.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18443397699866756 2 0.12699527456938475 3 0.1271837747648864 4 0.14969608382765265 5 0.1499922984205838 6 0.12387155704392916 7 0.12311755626192263 8 0.09998588941393673 9 0.11759719339366058 10 0.12037083912747029 11 0.13806292890526625 12 0.1408096460397186 13 0.15594351887856386 14 0.15720916304836052 15 0.14253307639859067 16 0.14387950636645946 17 0.129445777110906 18 0.12842249033532568 19 0.167630530999665 20 0.14519900773497088 21 0.15720916304836052 22 0.15063858480516082 23 0.16843838898038627 24 0.1535738021351148 25 0.17877897113361862 26 0.16321424070505536 27 0.17665161178438593 28 0.17643618298952693 29 0.17856354233875962 30 0.17018874793861571 31 0.17708246937410396 32 0.16472224226906843 33 0.17783647015611048 34 0.1926741284020246 35 0.1812564022744972 36 0.18287211823593977 37 0.20088735120602422 38 0.1948014877512573 39 0.16633795823051098 40 0.1949361307480442 41 0.18712683693440516 42 0.23979917727743244 43 0.20161442338867339 44 0.18481097738967084 45 0.17899439992847765 46 0.21190114834319101 47 0.19205477061680495 48 0.2241805896501544 49 0.2314513114766459 50 0.21971044215683 51 0.23220531225865243 52 0.21658672463137438 53 0.20737714365115187 54 0.19006205426435913 55 0.1924586996071656 56 0.21480943707378758 57 0.19399362977053602 58 0.2195757991600431 59 0.23163981167214753 60 0.2339018140181671 61 0.22084144332983982 62 0.23575988737382603 63 0.25302111956190404 64 0.23344402782909174 65 0.2582183392378775 66 0.2202759427433349 67 0.24179189362987827 68 0.21478250847443023 69 0.20810421583380098 70 0.2193872989645415 71 0.2142170078879253 72 0.21195500554190574 73 0.2064346426736437 74 0.2041995689269815 75 0.23209759786122294 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3713524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.14091408447594 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61474457813136 43.38169453952253 2 12.79738766811816 14.881036338355797 3 5.1944424934824385 9.060289041909405 4 2.5884008598572343 6.019679680365725 5 1.435609272305579 4.1733817679997856 6 0.9040691843210885 3.1538045263220793 7 0.5786869014640739 2.3551769793884016 8 0.39753872112437166 1.8490611704115627 9 0.27756921521573175 1.4524315104917944 >10 1.1633427451091714 11.04199799200841 >50 0.03680242697897777 1.428231766322709 >100 0.011173440985498299 1.0447372745219965 >500 1.394957439962962E-4 0.06512659279553959 >1k 9.299716238026257E-5 0.09335081958429886 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.6928599357375905E-5 2 0.0 0.0 0.0 0.0 2.6928599357375905E-5 3 0.0 0.0 0.0 0.0 2.6928599357375905E-5 4 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 5 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 6 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 7 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 8 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 9 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 10 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 11 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 12 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 13 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 14 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 15 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 16 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 17 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 18 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 19 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 20 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 21 0.0 0.0 0.0 2.6928599357375905E-5 2.6928599357375905E-5 22 0.0 0.0 0.0 8.078579807212772E-5 2.6928599357375905E-5 23 0.0 0.0 0.0 1.0771439742950362E-4 2.6928599357375905E-5 24 0.0 0.0 0.0 1.8850019550163132E-4 2.6928599357375905E-5 25 0.0 0.0 0.0 1.8850019550163132E-4 2.6928599357375905E-5 26 0.0 0.0 0.0 3.500717916458868E-4 2.6928599357375905E-5 27 0.0 0.0 0.0 5.385719871475181E-4 2.6928599357375905E-5 28 0.0 0.0 0.0 0.001157929772367164 2.6928599357375905E-5 29 0.0 0.0 0.0 0.001992716352445817 2.6928599357375905E-5 30 2.6928599357375905E-5 0.0 0.0 0.003716146711317875 2.6928599357375905E-5 31 2.6928599357375905E-5 0.0 0.0 0.008428651598858658 2.6928599357375905E-5 32 2.6928599357375905E-5 0.0 0.0 0.01276415609539618 2.6928599357375905E-5 33 2.6928599357375905E-5 0.0 0.0 0.01841916196044512 2.6928599357375905E-5 34 2.6928599357375905E-5 0.0 0.0 0.026336170171513636 2.6928599357375905E-5 35 2.6928599357375905E-5 0.0 0.0 0.03603046594016896 2.6928599357375905E-5 36 2.6928599357375905E-5 0.0 0.0 0.04777133525998486 2.6928599357375905E-5 37 2.6928599357375905E-5 0.0 0.0 0.0624743505091121 2.6928599357375905E-5 38 2.6928599357375905E-5 0.0 0.0 0.07933165370682942 2.6928599357375905E-5 39 2.6928599357375905E-5 0.0 0.0 0.09853174504863843 2.6928599357375905E-5 40 2.6928599357375905E-5 0.0 0.0 0.1160084060315754 2.6928599357375905E-5 41 2.6928599357375905E-5 0.0 0.0 0.13351199561386973 2.6928599357375905E-5 42 2.6928599357375905E-5 0.0 0.0 0.15147337138523947 2.6928599357375905E-5 43 2.6928599357375905E-5 0.0 0.0 0.17029646233604523 2.6928599357375905E-5 44 2.6928599357375905E-5 0.0 0.0 0.19286262859752623 2.6928599357375905E-5 45 2.6928599357375905E-5 0.0 0.0 0.21868715538124972 5.385719871475181E-5 46 2.6928599357375905E-5 0.0 0.0 0.24680061311035018 5.385719871475181E-5 47 2.6928599357375905E-5 0.0 0.0 0.2773915019803292 5.385719871475181E-5 48 2.6928599357375905E-5 0.0 0.0 0.3082247482445246 5.385719871475181E-5 49 2.6928599357375905E-5 0.0 0.0 0.34078142486759205 5.385719871475181E-5 50 2.6928599357375905E-5 0.0 0.0 0.37121074214142685 5.385719871475181E-5 51 2.6928599357375905E-5 0.0 0.0 0.4023402029985534 5.385719871475181E-5 52 2.6928599357375905E-5 0.0 0.0 0.43454680782997496 5.385719871475181E-5 53 2.6928599357375905E-5 0.0 0.0 0.4691769866035604 5.385719871475181E-5 54 2.6928599357375905E-5 0.0 0.0 0.507334811892962 5.385719871475181E-5 55 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.5491279980956094 5.385719871475181E-5 56 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.5945565452115026 5.385719871475181E-5 57 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.6439974536316447 5.385719871475181E-5 58 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.6971275801637474 5.385719871475181E-5 59 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.751684922461791 5.385719871475181E-5 60 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.8076964091251329 5.385719871475181E-5 61 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.8630616094038978 5.385719871475181E-5 62 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.9192346676633839 5.385719871475181E-5 63 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.976915727486883 5.385719871475181E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4060 0.0 39.61738 1 GTATCAA 8085 0.0 33.641243 1 ATCAACG 10125 0.0 26.50794 3 TATCAAC 10295 0.0 26.504766 2 TCAACGC 10130 0.0 26.393047 4 CAACGCA 10480 0.0 25.511255 5 AACGCAG 10735 0.0 24.82491 6 ACGCAGA 12430 0.0 21.303026 7 CGCAGAG 12645 0.0 20.778618 8 GTACATG 10225 0.0 19.91657 1 AGAGTAC 12515 0.0 19.756226 11 GTGGTAT 1895 0.0 19.307344 1 TACATGG 10300 0.0 18.922785 2 GAGTACT 8000 0.0 18.879904 12 CAGAGTA 13420 0.0 18.809114 10 GCAGAGT 13780 0.0 18.44285 9 AGTACTT 8515 0.0 17.780676 13 ACATGGG 10870 0.0 17.423452 3 GTACTTT 8555 0.0 17.374798 14 TGGTATC 2145 0.0 15.438951 2 >>END_MODULE