FastQCFastQC Report
Mon 6 Feb 2017
SRR2077290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713524
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.11738176459880156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55200.041.980791
GTATCAA107350.033.346721
ATCAACG148100.024.1602123
TATCAAC147900.024.1425512
TCAACGC149200.023.9582714
CAACGCA153900.023.2704325
GTGGTAT23450.023.1564331
AACGCAG159000.022.4733096
TGGTATC24050.021.2714752
ACGCAGA179400.019.8205247
CGCAGAG185150.019.242538
AGAGTAC183950.018.46796611
GAGTACT137700.018.27752512
GTACATG101050.017.9695761
CAGAGTA192200.017.89079910
AGTACTT142850.017.58706913
GCAGAGT199050.017.5694969
GTACTTT144600.017.29586814
TACATGG101800.017.045392
ACATGGG105450.015.867713
Produced by FastQC (version 0.11.5)