##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077290_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3713524 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92522035672854 31.0 31.0 34.0 27.0 34.0 2 31.023057613199754 33.0 31.0 34.0 27.0 34.0 3 31.094030898952045 33.0 31.0 34.0 27.0 34.0 4 34.444813605620965 37.0 35.0 37.0 31.0 37.0 5 34.16699797820076 37.0 35.0 37.0 30.0 37.0 6 34.149915282626424 37.0 35.0 37.0 30.0 37.0 7 33.98430358872058 37.0 35.0 37.0 30.0 37.0 8 33.943047628075114 37.0 35.0 37.0 29.0 37.0 9 35.404933157830676 39.0 37.0 39.0 29.0 39.0 10 35.30512634360247 39.0 35.0 39.0 28.0 39.0 11 35.28786915070429 39.0 35.0 39.0 28.0 39.0 12 35.18888204304052 39.0 35.0 39.0 27.0 39.0 13 35.110312737981495 39.0 35.0 39.0 27.0 39.0 14 36.35367780038583 40.0 37.0 41.0 27.0 41.0 15 36.40338314765167 40.0 37.0 41.0 27.0 41.0 16 36.38812728825773 40.0 37.0 41.0 27.0 41.0 17 36.39937051706142 40.0 37.0 41.0 27.0 41.0 18 36.40986163008506 40.0 37.0 41.0 28.0 41.0 19 36.45690750887836 40.0 37.0 41.0 27.0 41.0 20 36.428742078952496 40.0 37.0 41.0 27.0 41.0 21 36.41096139408282 40.0 37.0 41.0 27.0 41.0 22 36.394659089317855 40.0 37.0 41.0 27.0 41.0 23 36.368292759115064 40.0 37.0 41.0 27.0 41.0 24 36.351459691656764 40.0 37.0 41.0 27.0 41.0 25 36.33000836940868 40.0 37.0 41.0 28.0 41.0 26 36.259900030267744 40.0 36.0 41.0 27.0 41.0 27 36.20787047559138 40.0 36.0 41.0 27.0 41.0 28 36.18655918205995 40.0 36.0 41.0 27.0 41.0 29 36.13012087709679 40.0 36.0 41.0 27.0 41.0 30 36.04848871314687 40.0 36.0 41.0 27.0 41.0 31 35.9498659494324 40.0 36.0 41.0 27.0 41.0 32 35.86708070285798 40.0 36.0 41.0 27.0 41.0 33 35.79677713137171 40.0 35.0 41.0 27.0 41.0 34 35.729051165415925 40.0 35.0 41.0 27.0 41.0 35 35.64395867644857 40.0 35.0 41.0 26.0 41.0 36 35.581681712572745 40.0 35.0 41.0 26.0 41.0 37 35.51867740722828 40.0 35.0 41.0 25.0 41.0 38 35.459684655330086 40.0 35.0 41.0 25.0 41.0 39 35.38096508868665 40.0 35.0 41.0 25.0 41.0 40 35.313952999899826 40.0 35.0 41.0 25.0 41.0 41 35.256867062122126 40.0 35.0 41.0 25.0 41.0 42 35.18372602412156 40.0 35.0 41.0 24.0 41.0 43 35.0796679919128 40.0 35.0 41.0 24.0 41.0 44 34.945663202930696 40.0 35.0 41.0 23.0 41.0 45 34.84120528102148 39.0 35.0 41.0 23.0 41.0 46 34.69565727863883 39.0 35.0 41.0 22.0 41.0 47 34.52275520502897 39.0 34.0 41.0 20.0 41.0 48 34.403995234715055 39.0 34.0 41.0 19.0 41.0 49 34.302853031244716 39.0 34.0 41.0 18.0 41.0 50 34.29801557765616 39.0 34.0 41.0 17.0 41.0 51 34.22778956053603 39.0 34.0 41.0 15.0 41.0 52 34.15852812584488 39.0 34.0 41.0 15.0 41.0 53 34.065491700067106 39.0 34.0 41.0 14.0 41.0 54 34.03663016584786 39.0 34.0 41.0 14.0 41.0 55 33.95529744792278 39.0 34.0 41.0 13.0 41.0 56 33.83767655736169 39.0 34.0 41.0 11.0 41.0 57 33.762899606950164 39.0 34.0 41.0 9.0 41.0 58 33.67043164390482 38.0 34.0 41.0 8.0 41.0 59 33.58240420689351 38.0 33.0 41.0 8.0 41.0 60 33.55546133537847 38.0 34.0 41.0 7.0 41.0 61 33.44825588847682 38.0 34.0 41.0 6.0 41.0 62 33.30779389065481 38.0 33.0 40.0 2.0 41.0 63 33.18047439574916 37.0 33.0 40.0 2.0 41.0 64 33.03498779057305 37.0 33.0 40.0 2.0 41.0 65 32.908288730596595 37.0 33.0 40.0 2.0 41.0 66 32.71656437389391 37.0 33.0 40.0 2.0 41.0 67 32.53160124991787 36.0 33.0 40.0 2.0 41.0 68 32.3248994755386 36.0 33.0 39.0 2.0 41.0 69 32.16275322308406 36.0 33.0 39.0 2.0 41.0 70 31.97673692158715 36.0 32.0 39.0 2.0 41.0 71 31.800896129929416 36.0 32.0 39.0 2.0 41.0 72 31.602047812266733 35.0 32.0 39.0 2.0 41.0 73 31.407728077157977 35.0 32.0 39.0 2.0 40.0 74 31.14435883543502 35.0 32.0 38.0 2.0 40.0 75 30.920930092278923 35.0 32.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6278405249331875 1101 2 0.6336044559882801 1101 3 0.6221846447159081 1101 4 0.6376062238471008 1101 5 0.6273702616337431 1101 6 0.653911925866133 1101 7 0.6655213276509144 1101 8 0.6463151901794291 1101 9 0.8879115652170029 1101 10 0.815591357889268 1101 11 0.8966779423658622 1101 12 1.0077798984090904 1101 13 0.9835161236090713 1101 14 1.0627225600168089 1101 15 0.9381162229049238 1101 16 0.9841594330434305 1101 17 0.9352550031037978 1101 18 0.9550936566753592 1101 19 1.0187029756102532 1101 20 1.0339328750369035 1101 21 0.9597678096639797 1101 22 0.9785036281883492 1101 23 0.9208099369219127 1101 24 1.0454008227970135 1101 25 1.0910694475614022 1101 26 1.070900385240229 1101 27 1.1231809207736774 1101 28 1.0734913904555299 1101 29 1.0447468892270777 1101 30 1.0385925563903413 1101 31 1.1030492297574384 1101 32 1.0171352384349532 1101 33 1.0003846963433105 1101 34 0.9942470009951592 1101 35 1.1168945596361226 1101 36 1.0313420556907502 1101 37 1.095020458384532 1101 38 1.0598517456987508 1101 39 1.1079177854645081 1101 40 1.0862528090963792 1101 41 1.0431165046786788 1101 42 1.103146231813902 1101 43 1.100894427610477 1101 44 1.1526809390206623 1101 45 1.163051767962294 1101 46 1.1522128114479173 1101 47 1.1450418406216087 1101 48 1.1484400619114012 1101 49 1.0451323246133697 1101 50 0.8824004121421467 1101 51 0.9462350303386984 1101 52 0.9258459376520705 1101 53 0.9451946093886576 1101 54 0.9238928012531105 1101 55 0.9607910827934063 1101 56 1.0018005415754843 1101 57 1.0394793010498233 1101 58 0.9973254672584915 1101 59 0.9881142133956473 1101 60 1.0279606982967309 1101 61 0.9624997536014135 1101 62 0.9859628295292779 1101 63 0.9860571866427321 1101 64 1.0137401317039618 1101 65 0.9742500347268717 1101 66 1.0554596810687258 1101 67 1.0725506679627443 1101 68 1.1344081967578035 1101 69 1.1434970362129349 1101 70 1.1465704501282232 1101 71 1.079630677863502 1101 72 1.024440028959539 1101 73 1.0636075458035812 1101 74 1.1517631565006674 1101 75 1.2442036321693273 1102 1 0.7462897569578004 1102 2 0.8201597141350199 1102 3 0.7843363841944964 1102 4 0.7057713808433803 1102 5 0.7901333105363193 1102 6 0.9202987644733298 1102 7 1.0381859848116264 1102 8 0.9868389321873252 1102 9 1.1566588836373057 1102 10 1.1489415702014156 1102 11 1.1692424872178506 1102 12 1.1644203427654531 1102 13 1.1327510158340672 1102 14 1.352302821557494 1102 15 1.3323187837182502 1102 16 1.3213741329279287 1102 17 1.378333932112625 1102 18 1.2782600732427483 1102 19 1.3470376265929005 1102 20 1.3335402663598686 1102 21 1.3148450064902946 1102 22 1.4041570987604857 1102 23 1.4230351671112587 1102 24 1.3585962639481792 1102 25 1.4046920539225596 1102 26 1.363995721342441 1102 27 1.3691969372911217 1102 28 1.4189158078403707 1102 29 1.4056779699971713 1102 30 1.3836287572625352 1102 31 1.4408509188168779 1102 32 1.4253385310336881 1102 33 1.385228752728537 1102 34 1.3861346007898518 1102 35 1.4309552208710272 1102 36 1.4396298520801736 1102 37 1.3881594257394951 1102 38 1.4861660373660541 1102 39 1.4496633477450374 1102 40 1.4051910596210817 1102 41 1.3909130901157098 1102 42 1.4284840110339516 1102 43 1.3426357066232697 1102 44 1.3723180155670107 1102 45 1.446286658735339 1102 46 1.3773159547130618 1102 47 1.438677086252497 1102 48 1.5117510060960697 1102 49 1.498008321108351 1102 50 1.48000283487454 1102 51 1.4846482466154072 1102 52 1.4682505786815625 1102 53 1.3742275782086466 1102 54 1.4336405022636 1102 55 1.4073413099154948 1102 56 1.4300674588496136 1102 57 1.4792678455591428 1102 58 1.4402116855178448 1102 59 1.477918439020769 1102 60 1.5454080449575613 1102 61 1.5085062235136348 1102 62 1.5878759982544537 1102 63 1.5131062765209364 1102 64 1.500582513984888 1102 65 1.472350841070174 1102 66 1.5110409241311018 1102 67 1.4052224905222062 1102 68 1.4209595709244667 1102 69 1.4541296675985436 1102 70 1.4861301302044225 1102 71 1.3990469483279036 1102 72 1.4270738030338634 1102 73 1.445060052713071 1102 74 1.5374236472760323 1102 75 1.5315261034260566 1103 1 0.5508738813729188 1103 2 0.5644436943398183 1103 3 0.5619640725757087 1103 4 0.609668171904417 1103 5 0.5628998661921614 1103 6 0.6192970733739074 1103 7 0.6823086362065283 1103 8 0.6781069455086239 1103 9 0.8173579405640652 1103 10 0.8469428566819559 1103 11 0.8078104476274461 1103 12 0.8177662296589148 1103 13 0.7630502263085077 1103 14 0.9133888601311568 1103 15 0.8967278845305984 1103 16 0.9673450626176177 1103 17 0.9510833692773915 1103 18 0.9469991148147443 1103 19 0.9503238581386455 1103 20 0.9361619892063118 1103 21 0.9640751931337235 1103 22 1.0320099212365434 1103 23 1.0052449049389693 1103 24 1.0319816789937732 1103 25 0.9952384969076107 1103 26 1.085904796217683 1103 27 1.0947584756728546 1103 28 1.0596879036624216 1103 29 1.0349952015740413 1103 30 1.061784006414392 1103 31 1.1244716353035429 1103 32 1.0571981179074328 1103 33 1.0854310621686025 1103 34 1.147452517106366 1103 35 1.1642221552086411 1103 36 1.1761799055106152 1103 37 1.212369640809996 1103 38 1.1765112083953397 1103 39 1.197548954725228 1103 40 1.1223152568676014 1103 41 1.137093072893471 1103 42 1.19415469276489 1103 43 1.2172583217062112 1103 44 1.2176597110238632 1103 45 1.2658717687605474 1103 46 1.2233014091830654 1103 47 1.2949600912220447 1103 48 1.2361946551358756 1103 49 1.3019507614789418 1103 50 1.2839312692069669 1103 51 1.2021941812331463 1103 52 1.2259554558952956 1103 53 1.2116594933009281 1103 54 1.2215216480612696 1103 55 1.213001305558059 1103 56 1.1902814918623577 1103 57 1.1053486448370577 1103 58 1.1088635784079628 1103 59 1.180688522123262 1103 60 1.1501057739044143 1103 61 1.1728369942607273 1103 62 1.1528038412208303 1103 63 1.1802019429489832 1103 64 1.0963651402726597 1103 65 1.1121264332143426 1103 66 1.1216455699858372 1103 67 1.1722304895262212 1103 68 1.2433905004066617 1103 69 1.2651503446608032 1103 70 1.2339374071193632 1103 71 1.2606745537103698 1103 72 1.2536095391808502 1103 73 1.2176984790568746 1103 74 1.2845264723457994 1103 75 1.3157210674772237 1104 1 0.4029847167674454 1104 2 0.4256782320513004 1104 3 0.39182200726695626 1104 4 0.47502128734592475 1104 5 0.5717609250774771 1104 6 0.49657290443224156 1104 7 0.5771313335164834 1104 8 0.6035289054660211 1104 9 0.7258384637930746 1104 10 0.7385683663010809 1104 11 0.7972959244011122 1104 12 0.7395799659571551 1104 13 0.7613766845441319 1104 14 0.8325556169959043 1104 15 0.8403331416752877 1104 16 0.8257984596107448 1104 17 0.8678765805025748 1104 18 0.8115806692416001 1104 19 0.867311262478573 1104 20 0.8728117721296158 1104 21 0.8992475984023613 1104 22 0.9141323287100249 1104 23 0.8847311567479466 1104 24 0.9498741709060141 1104 25 0.9552783778194254 1104 26 0.9690098950146435 1104 27 0.99654656899245 1104 28 0.9510499346825441 1104 29 0.9834819451295544 1104 30 0.9478101531659888 1104 31 0.9934571942576724 1104 32 0.9323276690684494 1104 33 1.0062271971476875 1104 34 1.069161289570701 1104 35 1.0944053040702073 1104 36 1.024112933105883 1104 37 1.0813178510797457 1104 38 1.03094942137772 1104 39 0.9856751799157522 1104 40 0.9708923989382541 1104 41 0.9507132494678956 1104 42 1.013315336204549 1104 43 0.9884348253081257 1104 44 1.030537390407794 1104 45 1.0495742460673796 1104 46 1.02103306122428 1104 47 1.0605903151938918 1104 48 1.071751304203893 1104 49 1.1040948699411217 1104 50 1.0051963339558228 1104 51 1.1334184163200618 1104 52 0.9866290618005351 1104 53 0.9900004634046127 1104 54 1.068261254904563 1104 55 1.0364874477930783 1104 56 1.0836928565525525 1104 57 1.093582305956872 1104 58 1.056673335300772 1104 59 1.0161239382971772 1104 60 0.9950327001010066 1104 61 0.9981733996736821 1104 62 0.9778442633210958 1104 63 0.9910265137063661 1104 64 0.9925546056720123 1104 65 0.9851495586645029 1104 66 0.9852491768398224 1104 67 1.043677587353045 1104 68 1.0766690133544259 1104 69 1.0409344665925673 1104 70 1.0738984950016857 1104 71 1.0486782431457051 1104 72 1.0221700647616956 1104 73 1.1040045070944409 1104 74 1.1343016452922576 1104 75 1.1805472257207263 1105 1 0.5246709471367446 1105 2 0.5883978291896135 1105 3 0.5674329103621822 1105 4 0.5569256919227854 1105 5 0.5941431791529297 1105 6 0.659546331998051 1105 7 0.6550230733715665 1105 8 0.6979767955723801 1105 9 0.8373318519507222 1105 10 0.8691494452782038 1105 11 0.7768827384910608 1105 12 0.7566988438454558 1105 13 0.9102305552986252 1105 14 1.0176001681282472 1105 15 0.9235024232164974 1105 16 0.9394170554652348 1105 17 0.9186387098705922 1105 18 0.8987850123070089 1105 19 0.9982365247955443 1105 20 0.9705535235978502 1105 21 0.9733561929041059 1105 22 1.017282557017026 1105 23 1.0951919948860578 1105 24 1.0244306376477184 1105 25 1.0213339499652037 1105 26 1.054472902643596 1105 27 1.1081086151541086 1105 28 1.040121443652751 1105 29 1.0635461110113766 1105 30 1.0668610264498568 1105 31 1.0099421341627774 1105 32 1.119392590443553 1105 33 1.0709113320440764 1105 34 1.1473269595477547 1105 35 1.0910289382481153 1105 36 1.186056937271303 1105 37 1.1757136734061078 1105 38 1.055260225186835 1105 39 1.1321188101630426 1105 40 1.0511524570806046 1105 41 1.0448962064583824 1105 42 1.0791488545826908 1105 43 1.1044978630547106 1105 44 1.1309619725255757 1105 45 1.1031887454127016 1105 46 1.0511165610979347 1105 47 1.124814454225067 1105 48 1.1322085690629962 1105 49 1.1487900820024706 1105 50 1.1629655996725106 1105 51 1.1140044085558358 1105 52 1.061879574183223 1105 53 1.1280640524571055 1105 54 1.1403523505484827 1105 55 1.178321037636799 1105 56 1.2051749633578623 1105 57 1.1938359449587068 1105 58 1.124074119380282 1105 59 1.1749202026892007 1105 60 1.2086405123098771 1105 61 1.2562213811538854 1105 62 1.258135396229214 1105 63 1.2082423167880592 1105 64 1.2634602660012106 1105 65 1.2455242552548285 1105 66 1.215532333678695 1105 67 1.233252928665003 1105 68 1.2662927853588073 1105 69 1.1157837507882107 1105 70 1.16585785150518 1105 71 1.0688069705223846 1105 72 1.049585346144653 1105 73 1.0439106957584734 1105 74 1.1371541428866756 1105 75 1.1746705855218487 1106 1 0.2628453420040522 1106 2 0.37597577083579736 1106 3 0.30319371803900097 1106 4 0.3113716639540627 1106 5 0.27773689660491385 1106 6 0.3807325726068598 1106 7 0.4232464898889461 1106 8 0.431997542978948 1106 9 0.524537617896641 1106 10 0.5309861601332884 1106 11 0.5393610398145015 1106 12 0.520910261862646 1106 13 0.5620338021149962 1106 14 0.53855032713615 1106 15 0.48724028931656704 1106 16 0.48456974471240954 1106 17 0.5276236978582034 1106 18 0.5391716306463863 1106 19 0.5962020568818076 1106 20 0.6435985596350093 1106 21 0.6192907288648826 1106 22 0.6505464545853883 1106 23 0.5926464797421218 1106 24 0.5958508767844961 1106 25 0.5915765585280255 1106 26 0.6761021434329422 1106 27 0.6231914520217217 1106 28 0.5882779240926936 1106 29 0.6173602175813855 1106 30 0.5008734415646359 1106 31 0.4580041819623588 1106 32 0.524655821428567 1106 33 0.558278188964735 1106 34 0.614075560417092 1106 35 0.5602994461012969 1106 36 0.544982904858692 1106 37 0.5343521769264967 1106 38 0.504205495916878 1106 39 0.60416952604578 1106 40 0.5604207429893151 1106 41 0.5452128335466568 1106 42 0.49769375869190213 1106 43 0.49458799067765113 1106 44 0.5822263978057478 1106 45 0.5275232036054263 1106 46 0.5837999756711127 1106 47 0.6096066329805012 1106 48 0.5119805420108179 1106 49 0.5432824371247449 1106 50 0.5119122591178566 1106 51 0.5186913226915735 1106 52 0.47277543442291403 1106 53 0.4084303955531041 1106 54 0.4777143469655982 1106 55 0.49294837588617924 1106 56 0.5784130308216504 1106 57 0.5767547467751442 1106 58 0.4510165849054246 1106 59 0.44808466824211735 1106 60 0.4240066470985866 1106 61 0.4904407148482832 1106 62 0.49101184222901395 1106 63 0.501140426746538 1106 64 0.49477579600440436 1106 65 0.51958166474531 1106 66 0.47151700226178406 1106 67 0.46729450680106766 1106 68 0.4941559691275046 1106 69 0.3997788069617414 1106 70 0.47123634586004925 1106 71 0.4729870035738095 1106 72 0.4796468662324216 1106 73 0.41079677944245674 1106 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-1.18143140997649 2216 8 -1.1621734449668395 2216 9 -1.6848886551968505 2216 10 -1.9278668965247405 2216 11 -1.8806834562442347 2216 12 -1.6967348785239835 2216 13 -1.8085060635460408 2216 14 -1.97858178985814 2216 15 -1.7595483515684762 2216 16 -1.8030147488790504 2216 17 -2.121377125985333 2216 18 -1.8705477379009423 2216 19 -2.1488136897317105 2216 20 -2.235474963792065 2216 21 -2.2510164470817173 2216 22 -2.3263277894566343 2216 23 -2.3915136039751275 2216 24 -2.4708211781875633 2216 25 -2.373413919769156 2216 26 -2.412461887359811 2216 27 -2.316853724665066 2216 28 -2.1477872800744535 2216 29 -2.295057132194785 2216 30 -2.3431138477065403 2216 31 -2.3175302174046593 2216 32 -2.307873094628725 2216 33 -2.1394945975406756 2216 34 -2.2656961739508006 2216 35 -2.267658343615828 2216 36 -2.275708046049594 2216 37 -2.2255486215352818 2216 38 -2.2699833868747135 2216 39 -2.1713210578905446 2216 40 -2.236334929974582 2216 41 -2.1565993386352176 2216 42 -2.126948521638944 2216 43 -2.175575827986023 2216 44 -2.2617155162621287 2216 45 -2.274784550875019 2216 46 -2.072355950543134 2216 47 -2.2659341472744785 2216 48 -2.3132500561903413 2216 49 -2.268995242103763 2216 50 -2.2046192036227197 2216 51 -2.1750182561556457 2216 52 -2.20802886941512 2216 53 -2.2800331008048254 2216 54 -2.369023105018222 2216 55 -2.250662866387625 2216 56 -2.444878061033357 2216 57 -2.389756793415234 2216 58 -2.3141643274481183 2216 59 -2.308953522081339 2216 60 -2.2584629267006378 2216 61 -2.3367310912691437 2216 62 -2.2581100766883324 2216 63 -2.172808109265066 2216 64 -2.177709815093234 2216 65 -2.1080178026632446 2216 66 -2.2064154765997515 2216 67 -2.2299604351966593 2216 68 -2.246766650553141 2216 69 -2.3405070001000574 2216 70 -2.1335513052783703 2216 71 -2.0449353509454937 2216 72 -2.133502016899488 2216 73 -2.136507153595087 2216 74 -2.152276423745402 2216 75 -2.1413965196322344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79222.0 3 30838.0 4 17832.0 5 11466.0 6 8561.0 7 7641.0 8 7415.0 9 7723.0 10 8413.0 11 9038.0 12 9758.0 13 10039.0 14 10597.0 15 11307.0 16 12032.0 17 13379.0 18 14286.0 19 15879.0 20 18358.0 21 21149.0 22 27026.0 23 33549.0 24 35323.0 25 36535.0 26 36970.0 27 37149.0 28 41636.0 29 50389.0 30 57399.0 31 64553.0 32 77021.0 33 95852.0 34 124029.0 35 168843.0 36 236262.0 37 361362.0 38 609537.0 39 1007143.0 40 288013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.8863048399267 18.76643850382667 15.023013905357335 25.324242750889297 2 14.716194487911164 19.93037843428236 39.10924795242571 26.24417912538076 3 17.71839450814867 24.388213696828455 29.958994772755855 27.93439702226702 4 12.160036502600029 16.796724258399358 36.79411384787725 34.249125391123364 5 13.442399803771998 37.59437407849659 34.43046709272582 14.532759025005593 6 32.10834338019835 37.32653326298282 16.70352163951902 13.861601717299813 7 27.652206517733813 32.73587619624313 21.047037325156175 18.564879960866875 8 26.166661773337207 35.49219846159109 19.176724703349755 19.164415061721943 9 25.840237649285175 16.129830955619116 19.455649289487695 38.57428210560801 10 14.317483978835444 29.863111334265156 33.24191978683521 22.57748490006419 11 34.478513463542356 23.52790353123242 23.007024328075254 18.98655867714997 12 23.20645896093218 25.520470673864253 30.08031917402937 21.192751191174192 13 27.848430151717007 22.487947619885706 25.98521991567714 23.67840231272015 14 21.58216358844737 21.731364321896407 27.755862554279915 28.930609535376306 15 23.262439045411757 29.249314197756142 24.61166153353058 22.87658522330152 16 22.52643442479435 28.87339005504265 25.998264192863154 22.601911327299852 17 21.541139640259615 28.23725810609058 27.38168890679723 22.839913346852576 18 21.408098064068508 27.401922734037953 29.05305860368807 22.13692059820547 19 22.263598886412034 27.004184977119845 29.57156704083416 21.160649095633957 20 22.494865440333264 26.331309314061613 29.46221917473231 21.71160607087281 21 22.705235544414894 26.19918993181733 29.422950998066817 21.672623525700956 22 22.272415954227196 26.20535655370249 29.40009647323272 22.1221310188376 23 22.111961631253465 26.208005231318314 29.221992220891767 22.458040916536447 24 21.845656103368892 26.300130819085847 29.568938049069143 22.28527502847611 25 22.165263035989863 26.19209855130232 29.466790915138475 22.175847497569347 26 22.017215533577833 26.285380265034636 29.45030701618519 22.247097185202342 27 22.02776096511253 26.58299211928202 29.27609826210465 22.1131486535008 28 21.951027658433315 26.48763784700999 29.386260407302778 22.175074087253922 29 22.020540563841713 26.468741917406675 29.444456418656777 22.066261100094835 30 22.271718701722637 26.497081437706765 29.201516160045344 22.029683700525258 31 22.227596997104893 26.13565387144047 29.38413070183637 22.252618429618266 32 22.04034364816461 26.53343567369583 29.2368640758396 22.189356602299963 33 22.054397914360585 26.313610790167548 29.262700612171795 22.369290683300072 34 22.2058625103722 26.458187619830696 29.367196949355122 21.968752920441975 35 22.398836186269445 26.418404716655687 29.117183622477956 22.065575474596915 36 22.30621771956085 26.3311700378276 29.15453190569116 22.208080336920393 37 22.259000396687217 26.15356400439184 29.264959861339907 22.322475737581037 38 22.119047753693263 26.252776729785854 29.262753164105305 22.365422352415585 39 22.265679492710653 26.677180435556576 29.001897541383155 22.05524253034962 40 22.481623300690575 26.411384908564912 29.12413960635863 21.98285218438588 41 22.259450442523875 26.435708066476476 29.042940287891657 22.261901203107996 42 22.434930187077143 26.528722173194 28.700036436779225 22.33631120294963 43 22.204255928831934 26.31109205558874 29.034915594030476 22.449736421548852 44 22.299250887107373 26.646561772189536 28.62297451895835 22.431212821744744 45 22.41916884932721 26.701196060277514 28.48825779792845 22.391377292466817 46 22.59911333150754 26.49355006973298 28.27458878547206 22.632747813287423 47 22.476575651496216 26.692540967867984 28.191166058992223 22.63971732164358 48 22.359398391354777 26.891553117958612 28.14200049497605 22.607047995710566 49 22.57191583189106 26.790688931897442 27.94252173100491 22.69487350520658 50 22.534043854360245 27.030722072513967 27.89830885475475 22.53692521837104 51 22.526807882675456 27.122952717950376 27.63075406763504 22.719485331739133 52 22.616446375934245 27.136046335647997 27.591420968130237 22.656086320287518 53 22.65142220704646 27.078376227001627 27.67872242107497 22.591479144876942 54 22.498548548494636 26.973893261495014 27.819020423726897 22.708537766283456 55 22.390490516529628 26.872875664397654 27.872090962899293 22.864542856173426 56 22.37097665520128 27.27994475929682 27.745364919352667 22.603713666149233 57 22.589043685547647 27.054167580025002 27.6084840800969 22.74830465433045 58 22.417413151690514 26.950422981999534 27.822184641774705 22.809979224535244 59 22.48341342502674 27.112815640448083 27.699041592742084 22.704729341783086 60 22.404241207215566 27.146919957096742 27.841795452287094 22.607043383400597 61 22.641041480745024 26.881347479266086 27.92430392822693 22.553307111761953 62 22.46602771760078 27.09677888542955 27.909053861396917 22.528139535572752 63 22.40243433120122 26.859755301131067 27.867183630797772 22.87062673686994 64 22.335741182515903 26.932208748728165 27.940952306057365 22.791097762698573 65 22.342760414071538 26.872337818165658 27.83363880109403 22.951262966668775 66 22.25012793234399 27.094077405801396 27.836946860944273 22.818847800910337 67 22.34691775149443 26.946327116811275 27.81910903848821 22.887646093206087 68 22.462942369280565 26.908861538842743 27.759375135506083 22.868820956370612 69 22.39006901395441 26.987911314631408 27.58955831015319 23.032461361261 70 22.3538682882187 26.80546061848873 27.824855789922182 23.01581530337039 71 22.418823526877134 26.75298416381473 27.69245311676938 23.135739192538757 72 22.16448087461129 27.025401584414197 27.705863689960296 23.104253851014217 73 22.402440104888317 26.81645864320064 27.636730084941007 23.14437116697004 74 22.324525716243414 27.018249524820696 27.515528000497735 23.14169675843815 75 22.39695551518001 27.064424127412938 27.46841295834165 23.070207399065403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1776.0 1 1156.0 2 701.5 3 887.0 4 907.0 5 720.0 6 685.5 7 1435.5 8 2033.0 9 3597.5 10 6299.5 11 7326.5 12 7216.0 13 6424.0 14 5844.5 15 6648.5 16 7240.0 17 7983.5 18 9442.0 19 11313.0 20 12469.0 21 14230.5 22 18598.0 23 23608.0 24 26012.0 25 29911.0 26 38545.0 27 47434.0 28 51588.0 29 56462.5 30 65769.5 31 75528.5 32 80855.0 33 85850.5 34 96666.0 35 108368.5 36 114251.0 37 118348.0 38 127006.5 39 136411.0 40 141254.0 41 146122.5 42 154538.0 43 161714.0 44 165343.0 45 168789.5 46 174489.0 47 177844.5 48 178947.0 49 178048.5 50 174766.5 51 170478.5 52 168574.0 53 165594.0 54 157123.0 55 144616.0 56 137600.0 57 130542.0 58 114955.5 59 96934.5 60 87442.0 61 77777.5 62 60306.0 63 44799.5 64 37100.0 65 31536.5 66 21740.5 67 14907.0 68 12306.0 69 10290.5 70 6893.0 71 4663.0 72 3815.0 73 3344.0 74 2579.0 75 1763.5 76 1242.0 77 1046.5 78 619.5 79 314.0 80 240.0 81 173.0 82 71.0 83 23.0 84 10.0 85 11.0 86 9.5 87 5.0 88 3.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07335350464949197 2 0.015995588018281288 3 0.002558216938950711 4 0.14258693359730543 5 0.09570424211611396 6 0.06952964354074459 7 0.028759744113677467 8 0.02657852756573002 9 0.049440908420142156 10 0.02937910189889711 11 0.015376230233061642 12 0.10634103886227744 13 0.08781416250440283 14 0.04701733447797833 15 0.019361662937953276 16 0.030429317273834775 17 0.02130052209168434 18 0.01847301915915987 19 0.01295265629089781 20 0.012117869710819157 21 0.02684781355930378 22 0.015537801829205897 23 0.013518156877402704 24 0.020546521309677816 25 0.014379872056838734 26 0.03174881864234619 27 0.02814038632845782 28 0.03627282333438535 29 0.049656337215001164 30 0.03368767779607726 31 0.019038519745664766 32 0.011417726127527384 33 0.013841300069691217 34 0.012737227496038804 35 0.005789648861835819 36 0.009748152967370078 37 0.00729765042584887 38 0.001965787753088441 39 0.008024722608498019 40 0.01071758254423561 41 0.01052908234873398 42 0.006543649643842345 43 0.01071758254423561 44 0.0089402949866488 45 0.0044701474933244 46 0.001965787753088441 47 0.005035648079829294 48 0.005735791663121068 49 0.001373358567226171 50 1.3464299678687952E-4 51 0.0017234303588720581 52 0.0026390027370228387 53 0.0 54 0.0 55 2.6928599357375904E-4 56 0.00861715179436029 57 0.0032852891215998607 58 2.4235739421638315E-4 59 0.007540007820065254 60 0.001427215765940923 61 0.001131001173009788 62 0.023293238444130157 63 0.008267080002714403 64 0.010502153749376603 65 0.017207374989363203 66 0.016803445999002565 67 0.01532237303434689 68 0.015914802220209162 69 0.0058435060605505715 70 0.014487586454268236 71 0.0068937214354882325 72 0.0163187312105698 73 0.006489792445127593 74 0.01925394854052377 75 0.001481072964655675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3713524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.5593534472875 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03405787995678 42.61352504649372 2 13.18343857395043 15.176604010572333 3 5.297627227799566 9.147839941106673 4 2.5907217853076894 5.96481083696452 5 1.4606482121554616 4.203698335280239 6 0.9101541881203823 3.1432731963330047 7 0.591093362145956 2.3816066226473214 8 0.40342490689643196 1.8576701444392625 9 0.3033884294669945 1.5716557659157373 >10 1.176138928252788 11.067676568231704 >50 0.03799373400171227 1.4754461546032736 >100 0.010984130891339898 1.0409710392528035 >500 1.8779489017672256E-4 0.08458318240408068 >1k 1.4084616427909128E-4 0.2706391557554029 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4359 0.11738176459880156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.6928599357375905E-5 0.0 0.0 0.0 0.0 7 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 8 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 9 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 10 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 11 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 12 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 13 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 14 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 15 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 16 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 17 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 18 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 19 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 20 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 21 2.6928599357375905E-5 0.0 0.0 2.6928599357375905E-5 0.0 22 2.6928599357375905E-5 0.0 0.0 8.078579807212772E-5 0.0 23 2.6928599357375905E-5 2.6928599357375905E-5 0.0 1.0771439742950362E-4 0.0 24 2.6928599357375905E-5 2.6928599357375905E-5 0.0 1.8850019550163132E-4 0.0 25 2.6928599357375905E-5 2.6928599357375905E-5 0.0 1.8850019550163132E-4 0.0 26 2.6928599357375905E-5 2.6928599357375905E-5 0.0 3.500717916458868E-4 0.0 27 2.6928599357375905E-5 2.6928599357375905E-5 0.0 5.385719871475181E-4 0.0 28 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.001157929772367164 0.0 29 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.002019644951803193 0.0 30 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.0037700039100326266 0.0 31 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.008374794400143907 0.0 32 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.012548727300537172 0.0 33 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.01809601876815661 0.0 34 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.025959169780510372 0.0 35 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.035545751151736193 0.0 36 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.04725969187219471 0.0 37 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.06201656432003671 0.0 38 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.0786853673222524 2.6928599357375905E-5 39 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.09780467286598929 2.6928599357375905E-5 40 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.11506590505406725 2.6928599357375905E-5 41 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.13259642323571896 2.6928599357375905E-5 42 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.1506655134045182 2.6928599357375905E-5 43 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.16956939015339606 2.6928599357375905E-5 44 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.19224327081230658 5.385719871475181E-5 45 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.21839094078831858 5.385719871475181E-5 46 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.24647746991806166 5.385719871475181E-5 47 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.2770414301886833 5.385719871475181E-5 48 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.3079823908503082 5.385719871475181E-5 49 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.3405659960727331 5.385719871475181E-5 50 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.3708337417504236 5.385719871475181E-5 51 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.4021786314024091 5.385719871475181E-5 52 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.43438523623383074 5.385719871475181E-5 53 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.46912312940484563 5.385719871475181E-5 54 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.5074694548897489 5.385719871475181E-5 55 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.5495857842846849 5.385719871475181E-5 56 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.5956336891857976 5.385719871475181E-5 57 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.64558624099373 5.385719871475181E-5 58 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.6985547959296884 5.385719871475181E-5 59 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.7541354250033122 5.385719871475181E-5 60 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.8108201266505884 5.385719871475181E-5 61 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.8667777561152157 5.385719871475181E-5 62 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.9234893863618493 5.385719871475181E-5 63 2.6928599357375905E-5 2.6928599357375905E-5 0.0 0.9815474465763517 5.385719871475181E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5520 0.0 41.98079 1 GTATCAA 10735 0.0 33.34672 1 ATCAACG 14810 0.0 24.160212 3 TATCAAC 14790 0.0 24.142551 2 TCAACGC 14920 0.0 23.958271 4 CAACGCA 15390 0.0 23.270432 5 GTGGTAT 2345 0.0 23.156433 1 AACGCAG 15900 0.0 22.473309 6 TGGTATC 2405 0.0 21.271475 2 ACGCAGA 17940 0.0 19.820524 7 CGCAGAG 18515 0.0 19.24253 8 AGAGTAC 18395 0.0 18.467966 11 GAGTACT 13770 0.0 18.277525 12 GTACATG 10105 0.0 17.969576 1 CAGAGTA 19220 0.0 17.890799 10 AGTACTT 14285 0.0 17.587069 13 GCAGAGT 19905 0.0 17.569496 9 GTACTTT 14460 0.0 17.295868 14 TACATGG 10180 0.0 17.04539 2 ACATGGG 10545 0.0 15.86771 3 >>END_MODULE