FastQCFastQC Report
Mon 6 Feb 2017
SRR2077289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4546858
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.036.2523881
GTATCAA96200.034.2080461
ATCAACG123350.025.9841563
TCAACGC125200.025.6277624
TATCAAC127300.025.5559142
CAACGCA125950.025.4762785
AACGCAG132450.024.4096666
GTACATG156500.021.5350511
TACATGG159000.020.5692922
ACGCAGA155700.020.5451157
CGCAGAG157900.020.1089298
ACATGGG163750.019.3627173
AGAGTAC153550.019.0183911
GAGTACT79700.018.90441712
CAGAGTA164850.018.19536810
GTGGTAT25200.018.0877191
CATGGGG106600.017.994914
GCAGAGT172300.017.7684149
GTACTTT83700.017.67436214
AGTACTT84700.017.62737713
Produced by FastQC (version 0.11.5)