FastQCFastQC Report
Mon 6 Feb 2017
SRR2077289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4546858
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.10050896685139497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.040.8929371
GTATCAA118450.034.4275171
TATCAAC163100.024.9693682
ATCAACG165100.024.5635473
TCAACGC168200.024.1110954
CAACGCA169950.023.8286635
AACGCAG176900.022.911256
GTATACG1304.8194142E-621.2849961
GTGGTAT27850.020.8646451
GTACATG141650.019.9983541
ACGCAGA204700.019.5642437
TACATGG141950.019.2644792
CGCAGAG209600.019.0912428
TGGTATC28250.018.8459682
AGAGTAC206250.018.53086311
GAGTACT140650.018.36131912
ACATGGG148500.017.973433
AGTACTT144500.017.67376113
CAGAGTA218300.017.65031410
GCAGAGT223250.017.5991959
Produced by FastQC (version 0.11.5)