FastQCFastQC Report
Mon 6 Feb 2017
SRR2077288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4498777
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108000.034.5533521
GGTATCA54350.034.1720471
TCAACGC134650.027.184814
ATCAACG135250.027.0639113
TATCAAC137750.026.695582
CAACGCA138000.026.4504785
AACGCAG143300.025.5248076
ACGCAGA166150.021.7879337
CGCAGAG168700.021.3583168
GAGTACT89200.020.874412
AGAGTAC161300.020.65037311
GTACATG153250.019.6878911
CAGAGTA175050.019.57919710
AGTACTT97050.019.36467713
TACATGG155150.019.1881352
GTACTTT97300.019.10313214
GCAGAGT183050.019.0625699
ACATGGG160300.017.9276733
TAGTCGG2702.919478E-916.61474240
TATACCG2502.0396328E-816.5602995
Produced by FastQC (version 0.11.5)