FastQCFastQC Report
Mon 6 Feb 2017
SRR2077288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4498777
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.037.44021
GTATCAA129150.035.3530041
TATCAAC181800.024.9686432
TCAACGC181500.024.8235844
ATCAACG181950.024.8004743
CAACGCA186100.024.1561475
AACGCAG191850.023.444156
GTACATG148350.020.1685811
ACGCAGA223300.019.9562877
TACATGG147250.019.8157832
CGCAGAG225500.019.7165498
AGAGTAC217450.019.44787211
GAGTACT149950.019.15456212
AGTACTT157350.018.40179413
CAGAGTA234350.018.3842110
ACATGGG152200.018.3341353
GTACTTT160650.017.9742314
GCAGAGT241150.017.9075629
CTCGTAT7200.017.72329139
TTAGTCG2359.540599E-917.61118339
Produced by FastQC (version 0.11.5)