FastQCFastQC Report
Mon 6 Feb 2017
SRR2077287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4228810
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71600.033.028341
GGTATCA36050.030.3573041
TCAACGC88400.026.0696224
ATCAACG88900.026.0388013
CAACGCA89900.025.6727165
TATCAAC91350.025.4908982
AACGCAG93550.024.5412226
ACGCAGA109500.020.6551077
CGCAGAG112800.019.9918378
GTACATG97000.019.254611
AGAGTAC109300.019.15042111
TACATGG95850.018.8234622
GTACCGT3300.018.8028776
GTGTAGC23500.018.5102331
GAGTACT67300.018.39476812
GTGTACG5100.018.276911
GTATAGC12800.017.5312371
TGTACCG4750.017.4310285
CAGAGTA124150.017.41446110
ACATGGG101400.017.2152273
Produced by FastQC (version 0.11.5)