FastQCFastQC Report
Mon 6 Feb 2017
SRR2077287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4228810
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39550.035.9501151
GTATCAA80150.035.608641
TATCAAC106750.026.2685682
TCAACGC107350.026.0941814
ATCAACG108150.025.965733
CAACGCA110150.025.3648785
AACGCAG115450.024.4023276
ACGCAGA132950.020.9578117
CGCAGAG138050.020.0599378
AGAGTAC135300.019.49902511
GAGTACT98650.019.04228812
CCGCGTA1108.191711E-418.83522210
GTACATG92500.018.737041
TACATGG93650.018.054462
AGTACTT106000.018.00848613
CAGAGTA148250.018.0051610
GTACTTT105950.017.68343414
GCAGAGT155400.017.464789
ACATGGG94550.016.5694333
GTGGTAT21950.016.3909381
Produced by FastQC (version 0.11.5)